ZFIN ID: ZDB-FIG-081022-7
Krens et al., 2008 - ERK1 and ERK2 MAPK are key regulators of distinct gene sets in zebrafish embryogenesis. BMC Genomics   9:196 Full text @ BMC Genomics
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 30%-epiboly

Fig. 4 Quantitative real-time PCR confirmation of the microarray results. (A-G) qPCR was performed on seven genes that showed differential regulation of expression in response to knockdown of either ERK1, ERK2 and the standard control MO control:cdh2 (NM_131081), mycn (NM_212614), erm (NM_131205), cfos (NM_205569), mos (NM_205580), snai1a (NM_131066) and vegf (NM_131408), correlated to the β-actin housekeeping gene. A comparison of the fold changes in expression of these genes, detected by qPRC assay and microarray, are listed in a table (H). ⇑ = induction of expression, ⇓ = repression of expression, compared to the standard control MO (A-C, red line).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh2 WT control 30%-epiboly whole organism RTPCR
WT + MO1-mapk1 standard conditions 30%-epiboly whole organism RTPCR
WT + MO3-mapk3 standard conditions 30%-epiboly whole organism RTPCR
etv5b WT control 30%-epiboly whole organism RTPCR
WT + MO1-mapk1 standard conditions 30%-epiboly whole organism RTPCR
WT + MO3-mapk3 standard conditions 30%-epiboly whole organism RTPCR
fosab WT control 30%-epiboly whole organism RTPCR
WT + MO1-mapk1 standard conditions 30%-epiboly whole organism RTPCR
WT + MO3-mapk3 standard conditions 30%-epiboly whole organism RTPCR
mos WT control 30%-epiboly whole organism RTPCR
WT + MO1-mapk1 standard conditions 30%-epiboly whole organism RTPCR
WT + MO3-mapk3 standard conditions 30%-epiboly whole organism RTPCR
mycn WT control 30%-epiboly whole organism RTPCR
WT + MO1-mapk1 standard conditions 30%-epiboly whole organism RTPCR
WT + MO3-mapk3 standard conditions 30%-epiboly whole organism RTPCR
snai1a WT control 30%-epiboly whole organism RTPCR
WT + MO1-mapk1 standard conditions 30%-epiboly whole organism RTPCR
WT + MO3-mapk3 standard conditions 30%-epiboly whole organism RTPCR
vegfaa WT control 30%-epiboly whole organism RTPCR
WT + MO1-mapk1 standard conditions 30%-epiboly whole organism RTPCR
WT + MO3-mapk3 standard conditions 30%-epiboly whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Genomics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Genomics