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ZFIN ID: ZDB-FIG-080528-11
Krens et al., 2008 - ERK1 and ERK2 MAPK are key regulators of distinct gene sets in zebrafish embryogenesis. BMC Genomics   9:196 Full text @ BMC Genomics
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 30%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 30%-epiboly

Fig. 1 Phenotype and Function analysis of morpholino mediated knockdown of ERK1 and ERK2. Differential interference contrast (DIC) microscopy of 4.5 h old embryos using a 10x objective (A,B,C,) or an enlargement of the margin, using a 40x objective (D,E,F) Wild type (Wt) and ERK1 morphants are at approximately 30% epiboly stage and undergo epiboly, whereas ERK2 morphants do not initiate epiboly. Localization of active ERK (dpERK) was detected by immuno-localization in wild type, ERK1MO and ERK2MO injected embryos at 4.5 hpf (G-I) by phospho-specific ERK antibody. The level of dpERK was lower in ERK1 morphants compared to wild type embryos, whereas ERK2 morphants hardly showed any active ERK staining, (A-C); lateral view, animal pole to top, (G-I); animal pole view, dorsal to right. The bar graphs in (J) represent the quantification of a western blot analysis of zebrafish wild type, ERK1MO and ERK2MO injected embryos, probes with a global ERK1 antibody (Santacruz). This antibody recognizes both zebrafish ERK1 (45.7 kD) and ERK2 (43.3 kD) protein. The bars represent the maximum pixel-intensity measured in duplo and clearly show the specific knockdown of either ERK1 or ERK2 by the corresponding morpholino.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-mapk IHC WT standard conditions 30%-epiboly margin
IHC WT + MO1-mapk1 standard conditions 30%-epiboly margin
IHC WT + MO3-mapk3 standard conditions 30%-epiboly margin
Ab3-mapk WB WT standard conditions 30%-epiboly whole organism
WB WT + MO1-mapk1 standard conditions 30%-epiboly whole organism
WB WT + MO3-mapk3 standard conditions 30%-epiboly whole organism
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-mapk1 standard conditions 30%-epiboly gastrulation disrupted, abnormal
WT + MO3-mapk3 standard conditions 30%-epiboly gastrulation occurrence, normal
30%-epiboly margin disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Genomics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Genomics