FIGURE

Fig. 10

ID

ZDB-FIG-080528-12

Publication

Krens et al., 2008 - ERK1 and ERK2 MAPK are key regulators of distinct gene sets in zebrafish embryogenesis

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Fig. 10

Effects of ERK1 and ERK2 knockdown affect developmental signaling pathways confirmed by whole mount in situ hybridization. The zebrafish embryos were injected with 3.4 ng ERK1MO (B,E,H,K,N,Q) or ERK2MO (C,F,I,L,O,R) and in situ expression patterns, were compared to wild type embryos (A,D,G,J,M,P). The embryos were fixed at 4.5hpf, processed for whole mount in situ hybridization, and imaged (animal pole view, dorsal to right). A,B,C: goosecoid (gsc, presumptive shield/dorsal organizer); D,E,F: lft1/antivin, (blastula margin); G,H,I: vox (expressed in blastula, but the dorsal most region); J,H,L vent (ventral blastula margin); M,N,O, notail (ntl, blastula margin); P,Q,R,: tbx6 (margin).

Expression Data

Genes:	gsc lft1 tbx16l tbxta vent vox
Fish:	WT WT + MO1-mapk1 WT + MO3-mapk3
Knockdown Reagents:	MO1-mapk1 MO3-mapk3
Anatomical Terms:	embryonic structure margin presumptive shield
Stage:	30%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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