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ZFIN ID: ZDB-FIG-080528-12
Krens et al., 2008 - ERK1 and ERK2 MAPK are key regulators of distinct gene sets in zebrafish embryogenesis. BMC Genomics   9:196 Full text @ BMC Genomics
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 30%-epiboly

Fig. 10 Effects of ERK1 and ERK2 knockdown affect developmental signaling pathways confirmed by whole mount in situ hybridization. The zebrafish embryos were injected with 3.4 ng ERK1MO (B,E,H,K,N,Q) or ERK2MO (C,F,I,L,O,R) and in situ expression patterns, were compared to wild type embryos (A,D,G,J,M,P). The embryos were fixed at 4.5hpf, processed for whole mount in situ hybridization, and imaged (animal pole view, dorsal to right). A,B,C: goosecoid (gsc, presumptive shield/dorsal organizer); D,E,F: lft1/antivin, (blastula margin); G,H,I: vox (expressed in blastula, but the dorsal most region); J,H,L vent (ventral blastula margin); M,N,O, notail (ntl, blastula margin); P,Q,R,: tbx6 (margin).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gsc WT standard conditions 30%-epiboly presumptive shield ISH
WT + MO1-mapk1 standard conditions 30%-epiboly Not Detected whole organism ISH
WT + MO3-mapk3 standard conditions 30%-epiboly presumptive shield ISH
lft1 WT standard conditions 30%-epiboly margin ISH
WT + MO1-mapk1 standard conditions 30%-epiboly embryonic structure ISH
WT + MO3-mapk3 standard conditions 30%-epiboly margin ISH
tbx16l WT standard conditions 30%-epiboly margin ISH
WT + MO1-mapk1 standard conditions 30%-epiboly margin ISH
WT + MO3-mapk3 standard conditions 30%-epiboly margin ISH
tbxta WT standard conditions 30%-epiboly margin ISH
WT + MO1-mapk1 standard conditions 30%-epiboly embryonic structure ISH
WT + MO3-mapk3 standard conditions 30%-epiboly margin ISH
vent WT standard conditions 30%-epiboly embryonic structure ISH
WT + MO1-mapk1 standard conditions 30%-epiboly embryonic structure ISH
WT + MO3-mapk3 standard conditions 30%-epiboly embryonic structure ISH
vox WT standard conditions 30%-epiboly embryonic structure ISH
WT + MO1-mapk1 standard conditions 30%-epiboly embryonic structure ISH
WT + MO3-mapk3 standard conditions 30%-epiboly embryonic structure ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Genomics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Genomics