FIGURE

Fig. S3

ID

ZDB-FIG-080604-56

Publication

Elworthy et al., 2008 - Expression of multiple slow myosin heavy chain genes reveals a diversity of zebrafish slow twitch muscle fibres with differing requirements for Hedgehog and Prdm1 activity

Other Figures

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Fig. S3

smyhc1 expression in the craniofacial muscles is prdm1 independent. (A) Lateral views of wild-type sib and ubo (prdm1)^tp39 mutant head at 96 hpf with immunofluorescence to the general muscle MF20 antigen and cartilage Col2 II-II6B3 antigen. In the ubo (prdm1)^tp39 mutant, perturbations to the craniofacial muscles are associated with the major loss of skeletal elements in the posterior head mutant. The ceratobranchial cartilage is absent, and the sternohyoidus and inferior obliquus form abnormal attachments to the posterior basicranial commissure cartilage instead of the cleithrum. (B) Ventrolateral views of wild type and nrd (prdm1)^m805 mutants at 96 hpf with smyhc1 (1 to 249 bp), smyhc2 (1 to 155 bp) or smyhc3 (1 to 129 bp) ISH showing prdm1-independent expression. nrd (prdm1)^m805 mutants lack posterior transversus ventralis muscles. Scale bars: 25 µm. Abbreviations: bc, basicranial commissure; cb, ceratobranchial; iob, inferior obliquus; sh, sternohyoidus; tv, transversus ventralis.

Expression Data

Genes:	smyhc1 smyhc2 smyhc3
Antibodies:	Ab-MF20 Ab1-col2a
Fish:	WT prdm1a^m805/m805 prdm1a^tp39/tp39
Anatomical Terms:	cephalic musculature cranial cartilage muscle
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	prdm1a^m805/m805 prdm1a^tp39/tp39
Observed In:	cephalic musculature ceratobranchial cartilage muscle attachment ventral transverse
Stage:	Day 4

Phenotype Detail

Acknowledgments

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