Fig. S3

Fig. S3 smyhc1 expression in the craniofacial muscles is prdm1 independent. (A) Lateral views of wild-type sib and ubo (prdm1)^tp39 mutant head at 96 hpf with immunofluorescence to the general muscle MF20 antigen and cartilage Col2 II-II6B3 antigen. In the ubo (prdm1)^tp39 mutant, perturbations to the craniofacial muscles are associated with the major loss of skeletal elements in the posterior head mutant. The ceratobranchial cartilage is absent, and the sternohyoidus and inferior obliquus form abnormal attachments to the posterior basicranial commissure cartilage instead of the cleithrum. (B) Ventrolateral views of wild type and nrd (prdm1)^m805 mutants at 96 hpf with smyhc1 (1 to 249 bp), smyhc2 (1 to 155 bp) or smyhc3 (1 to 129 bp) ISH showing prdm1-independent expression. nrd (prdm1)^m805 mutants lack posterior transversus ventralis muscles. Scale bars: 25 µm. Abbreviations: bc, basicranial commissure; cb, ceratobranchial; iob, inferior obliquus; sh, sternohyoidus; tv, transversus ventralis.