ZFIN ID: ZDB-FIG-080604-48
Elworthy et al., 2008 - Expression of multiple slow myosin heavy chain genes reveals a diversity of zebrafish slow twitch muscle fibres with differing requirements for Hedgehog and Prdm1 activity. Development (Cambridge, England)   135(12):2115-2126 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Day 4

Fig. 3 Differential expression of Smyhc genes in craniofacial muscles. (A) Lateral and ventral views of the dorsal group of craniofacial muscles at 96 hpf with slow troponin C (stnnC), smyhc1 (1 to 249 bp), smyhc2 (1 to 324 bp) or smyhc3 (1 to 129 bp) in situ hybridisation (see Fig. 1A,D,E,G for gross location of these muscles). The lap and do lack smyhc1 expression while smyhc3 expression is restricted to the lap. (B) Ventral views showing ventral craniofacial muscles at 96 hpf with slow troponin C (stnnC), smyhc1 (1 to 249 bp), smyhc2 (1 to 324 bp) or smyhc3 (1 to 129 bp) in situ hybridisation. The ima, sh, iob and posterior tv lack smyhc1 expression. (C) Lateral and ventral views of the dorsal group of craniofacial muscles at 96 hpf showing colocalisation of slow MyHC S58 antigen with smyhc2 (1 to 324 bp) in situ hybridisation and, in the ao and lo, with smyhc1:gfp. (D) Ventral view at 96 hpf showing colocalisation of smyhc1:gfp and smyhc2 (1 to 324 bp) in situ hybridisation with slow MyHC S58 antigen. In the am, some fibres express smyhc2 and S58 antigen but not smyhc1:gfp (blue arrows). Some fibres express S58 antigen but neither smyhc2 nor smyhc1:gfp (white arrows). The extraocular muscles have zones of S58 antigen-expressing fibres that co-express smyhc1:gfp and zones that do not (pink arrows). (E) Ventral view at 96 hpf showing expression of slow troponin C in situ hybridisation (stnnC) in all slow MyHC S58 antigen-expressing fibres. Scale bars: 25 μm. Abbreviations: abh, abductor hyoideus; am, adductor mandibulae; ao, adductor operculi; do, dilator operculi; hh, hyohyoidus; ih, interhyoideus; ima, intermandibularis anterior; imp, intermandibularis posterior; io, inferior oblique; iob, inferior obliquus; ir, inferior rectus, lap, levator arcus palatini; lo, levatator operculi; sh, sternohyoideus; tv, transversus ventralis.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP i104Tg standard conditions Day 4 cephalic musculature IFL
i108Tg standard conditions Day 4 cephalic musculature IFL
smyhc1 WT standard conditions Day 4 abductor hyohyoid ISH
Day 4 adductor mandibulae ISH
Day 4 adductor operculi ISH
Day 4 Not Detected dilatator operculi ISH
Day 4 hyohyoideus ISH
Day 4 inferior hyohyoid ISH
Day 4 Not Detected levator arcus palatini ISH
Day 4 levator operculi ISH
Day 4 Not Detected sternohyoid ISH
Day 4 Not Detected ventral intermandibularis anterior ISH
Day 4 ventral intermandibularis posterior ISH
Day 4 Not Detected ventral transverse ISH
smyhc2 WT standard conditions Day 4 abductor hyohyoid ISH
Day 4 adductor mandibulae ISH
Day 4 adductor operculi ISH
Day 4 caudal oblique ISH
Day 4 dilatator operculi ISH
Day 4 hyohyoideus ISH
Day 4 inferior hyohyoid ISH
Day 4 levator arcus palatini ISH
Day 4 levator operculi ISH
Day 4 sternohyoid ISH
Day 4 ventral intermandibularis anterior ISH
Day 4 ventral intermandibularis posterior ISH
Day 4 ventral transverse ISH
smyhc3 WT standard conditions Day 4 levator arcus palatini ISH
tnnc1b WT standard conditions Day 4 abductor hyohyoid ISH
Day 4 dilatator operculi ISH
Day 4 hyohyoideus ISH
Day 4 inferior hyohyoid ISH
Day 4 levator arcus palatini ISH
Day 4 levator operculi ISH
Day 4 pectoral fin musculature ISH
Day 4 ventral intermandibularis anterior ISH
Day 4 ventral intermandibularis posterior ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-S58 IHC WT standard conditions Day 4 abductor hyohyoid
IHC Day 4 adductor mandibulae
IHC Day 4 adductor operculi
IHC Day 4 dilatator operculi
IHC Day 4 hyohyoideus
IHC Day 4 inferior hyohyoid
IHC Day 4 levator arcus palatini
IHC Day 4 levator operculi
IHC Day 4 pectoral fin musculature
IHC Day 4 slow muscle cell
IHC Day 4 ventral intermandibularis anterior
IHC Day 4 ventral intermandibularis posterior
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development