ZFIN ID: ZDB-FIG-080604-47
Elworthy et al., 2008 - Expression of multiple slow myosin heavy chain genes reveals a diversity of zebrafish slow twitch muscle fibres with differing requirements for Hedgehog and Prdm1 activity. Development (Cambridge, England)   135(12):2115-2126 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Days 30-44 to Adult

Fig. 2 Expression of smyhc1, smyhc2 and smyhc3 in the juvenile and adult trunk. All panels show trunk transverse cryosections. (A) At 32 dpf smyhc1 (1 to 249 bp) in situ hybridisation shows expression in the lateralis superficialis, while smyhc2 (1 to 324 bp) in situ hybridisation shows colocalisation with smyhc1:gfp in the lateralis superficialis. (B) At 42 dpf, smyhc1:gfp shows colocalisation with slow MyHC S58 antigen in the lateralis superficialis, which is not fast muscle F310 antigen positive. (C) At 42 dpf, smyhc2 (1 to 324 bp) and smyhc3 (1 to 129 bp) in situ hybridisation shows expression in the lateralis superficialis. smyhc1 (1 to 249 bp) in situ hybridisation on adjacent sections failed to detect any expression. (D) Adjacent sections of 22 month post-fertilisation adult with smyhc2 (1 to 324 bp) and smyhc3 (1 to 129 bp) in situ hybridisation showing expression in subsets of the slow MyHC S58 antigen positive, fast muscle F310 antigen negative, lateralis superficialis. smyhc1 (1 to 249 bp) in situ hybridisation on adjacent sections failed to detect any expression throughout the trunk. Scale bars: 25 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP i104Tg standard conditions Days 30-44 superficial lateralis IFL
i108Tg standard conditions Days 30-44 superficial lateralis IFL
smyhc1 WT standard conditions Days 30-44 superficial lateralis ISH
smyhc2 WT standard conditions Days 30-44 superficial lateralis ISH
Adult superficial lateralis ISH
smyhc3 WT standard conditions Days 30-44 superficial lateralis ISH
Adult superficial lateralis ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-F310 IHC WT standard conditions Days 30-44 fast muscle cell
IHC Days 30-44 Not Detected superficial lateralis
IHC Adult fast muscle cell
IHC Adult Not Detected superficial lateralis
Ab-S58 IHC WT standard conditions Days 30-44 slow muscle cell
IHC Days 30-44 superficial lateralis
IHC Adult slow muscle cell
IHC Adult superficial lateralis
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development