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ZFIN ID: ZDB-FIG-080325-119
Serluca, 2008 - Development of the proepicardial organ in the zebrafish. Developmental Biology   315(1):18-27 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 6 pandora/spt6 is required for the PEO lineage. (A) Buffer-injected control embryos shown as a live 48 hpf embryo, (B) stained with wt1 (blue)/MF20 (brown) to mark the PEO and myocardium respectively, and with tcf21 (C) as a second marker of the PEO. (D) spt6MO embryos shown as live 48 hpf embryos. Note the extensive pericardial edema (asterisk) and tail extension defects (arrow). The PEO is not visible either by (E) wt1 or (F) tcf21 staining. Myocardium is still present, however, as evidenced by the MF20 positive tissue in the wt1/MF20 double-stained spt6MO embryos (arrowhead in panel E). (G) hegMO embryos display an enlarged heart (arrow) but relatively a relatively normal PEO as assayed by (H) wt1, with two wt1-positive clusters at the characteristic positions (arrowheads) and (I) tcf21 expression. (J) sihMO embryos shown live at 48 hpf. Staining with (K) wt1 and (L) >wt1-positive clusters at the characteristic positions (arrowheads) and (I) tcf21 expression. (J) sihMO embryos shown live at 48 hpf. Staining with (K) wt1 and (L) tcf21 reveals no effect of heart function on the formation of the PEO (arrowhead in panel K). Anterior is to the left in all panels except B, C, I, K and L which are face views with dorsal at the top of the panel.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
tcf21 WT control Long-pec heart ISH
WT + MO1-heg1 standard conditions Long-pec heart ISH
WT + MO1-tnnt2a standard conditions Long-pec heart ISH
WT + MO2-supt6h standard conditions Long-pec Not Detected heart ISH
wt1a WT control Long-pec heart ISH
WT + MO1-heg1 standard conditions Long-pec heart ISH
WT + MO1-tnnt2a standard conditions Long-pec heart ISH
WT + MO2-supt6h standard conditions Long-pec Not Detected heart ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-heg1 standard conditions Long-pec endocardium morphology, normal
Long-pec heart increased size, abnormal
Long-pec pericardium edematous, abnormal
WT + MO1-tnnt2a standard conditions Long-pec endocardium morphology, normal
Long-pec pericardium edematous, abnormal
WT + MO2-supt6h standard conditions Long-pec heart decreased size, abnormal
Long-pec pericardium edematous, abnormal
Long-pec post-vent region decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 315(1), Serluca, F.C., Development of the proepicardial organ in the zebrafish, 18-27, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.