ZFIN ID: ZDB-FIG-080325-114
Serluca, 2008 - Development of the proepicardial organ in the zebrafish. Developmental Biology   315(1):18-27 Full text @ Dev. Biol.
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Day 4
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 4 wt1 is required for development of the PEO. Live 48 hpf embryos injected with buffer (A) as a control or with wt1MO (B). A slight pericardial edema is observed in the wt1MO embryos. tcf21 staining at 48 hpf of (C) buffer-injected or (D) wt1MO-injected embryos. The PEO is clearly visible in the control embryos (arrow) but is completely lacking in wt1MO embryos (127/127, 100%). (E) Control and (F) wt1MO-injected embryos shown at 4 dpf. wt1MO embryos display pericardial edema (arrow in panel F) as well as edema in the pronephros (arrowhead). (G) At 4 dpf, a fraction of the embryos (22/68, 32%) have tcf21-positive cells adjacent to the heart (arrow). (H) Same embryo as panel G but shown at higher magnification with the wt1-positive cells (arrow) nestled between the heart and yolk as observed in 48 hpf wild-type embryos. (I) Live view of a 4 dpf wt1MO embryo using DIC optics. The ventricle (v) and atrium (a) are marked and an arrow points to a PEO-like cluster on the surface of the ventricular myocardium. (J) Higher magnification view of the embryo in panel I.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
tcf21 WT standard conditions Long-pec heart ISH
WT + MO4-wt1a standard conditions Long-pec Not Detected heart ISH
Day 4 epicardium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO4-wt1a standard conditions Long-pec blood circulation occurrence, normal
Long-pec heart functional, normal
Long-pec pericardium edematous, abnormal
Day 4 blood circulation occurrence, normal
Day 4 coelom edematous, abnormal
Day 4 pericardium edematous, abnormal
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 315(1), Serluca, F.C., Development of the proepicardial organ in the zebrafish, 18-27, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.