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ZFIN ID: ZDB-FIG-071116-14
Blanco et al., 2007 - Snail1a and Snail1b cooperate in the anterior migration of the axial mesendoderm in the zebrafish embryo. Development (Cambridge, England)   134(22):4073-4081 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Bud
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Bud

Fig. 3 Snail1b modulates extension movements during gastrulation. (A,F,K) Knockdown of snail1b caused the posterior expansion of the spt expression domain, whereas overexpression of snail1b mRNA produced anterior compression. Similar effects were observed on the expression of hgg1 (B,G,L). (D,I,N) The expression of cdh1 follows that of hgg1 in the prechordal plate of control, as well as in snail1b morphants or overexpressing embryos. (C,H,M) Parasagittal sections obtained from hgg1-hybridised embryos reveal the position of the presumptive prechordal plate cells. Sections obtained from cdh1-hybridised embryos (E,J,O) confirm the similarity between hgg1 and cdh1 expression. (P-S) Increased cellular adhesion seems to account for the defects in extension movements in snail1b morphants, since the phenotype can be ameliorated by impairing cadherin activity with the injection of cdh1 MOs. The morphology of the polster is altered in the snail1b morphants (Q), although this effect can be reverted by the injection of cdh1 MOs (S). Asterisks indicate the defective posterior region where cells detach from the embryo both in the cdh1 (R) and in the double morphants (S). (T-W) Higher magnification of the images shown in P-S at the level of the prechordal plate to allow a better visualisation of the polster. The arrows indicate the territory it occupies along the anteroposterior axis. All embryos in this figure are at tail bud stages.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh1 WT standard conditions Bud prechordal plate ISH
WT + MO1-snai1b standard conditions Bud prechordal plate ISH
ctslb WT standard conditions Bud prechordal plate ISH
WT + MO1-snai1b standard conditions Bud prechordal plate ISH
dlx3b WT standard conditions Bud neural plate ISH
WT + MO1-snai1b standard conditions Bud neural plate ISH
tbx16 WT standard conditions Bud prechordal plate ISH
WT + MO1-snai1b standard conditions Bud prechordal plate ISH
tbxta WT standard conditions Bud axial chorda mesoderm ISH
WT + MO1-snai1b standard conditions Bud axial chorda mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-snai1b standard conditions Bud polster increased length, abnormal
Bud polster morphology, abnormal
WT + MO3-cdh1 + MO4-cdh1 standard conditions Bud polster decreased length, abnormal
Bud polster morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development