Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-FIG-070801-20
Jin et al., 2007 - A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos. Developmental Biology   307(1):29-42 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. S2 Endothelial fate specification appears unaffected in mutants with defective lumen formation. (A–H) In situ hybridization with the arterial specific marker ephrinB2a (A to D) and the venous specific marker flt4 (E to H) in 36 hpf logs231 mutant embryos (B and F) and wild-type siblings (A and E), and in 36 hpf mtss233 mutant embryos (D and H) and wild-type siblings (C and G). Black arrows point to the vascular expression of ephrinB2a and flt4. Although these mutants display defective vascular lumen formation, the specification of endothelial cells appears relatively unaffected.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
efnb2a med14s231/s231(TL) standard conditions Prim-25 artery ISH
mtss233/s233(TL) standard conditions Prim-25 artery ISH
flt4 med14s231/s231(TL) standard conditions Prim-25 vein ISH
mtss233/s233(TL) standard conditions Prim-25 vein ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sars1s277/s277 standard conditions Day 4 blood circulation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 307(1), Jin, S.W., Herzog, W., Santoro, M.M., Mitchell, T.S., Frantsve, J., Jungblut, B., Beis, D., Scott, I.C., D'Amico, L.A., Ober, E.A., Verkade, H., Field, H.A., Chi, N.C., Wehman, A.M., Baier, H., and Stainier, D.Y., A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos, 29-42, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.