ZFIN ID: ZDB-FIG-070801-13
Jin et al., 2007 - A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos. Developmental Biology   307(1):29-42 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-25

Fig. 4 Mutations affecting vascular tube formation. (A) Epifluorescent micrograph of 36 hpf Tg(flk1:EGFP)s843 embryo in lateral view (A). White line marks the location of the transverse sections shown in panels B to F. (B–G) Transverse sections of wild-type (B), sih/cmc2 MO injected (C), sols828 mutant (D), mtss233 mutant (E), logs231 mutant (F), and psns634 mutant (G) Tg(flk1:EGFP)s843 embryos. Lack of circulation does not affect vascular lumen formation (C). Note changes in the number of vascular lumens in sols828 and mtss233 mutant embryos, and in the size and shape of the vascular lumens in logs231 and psns634 mutant embryos.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tnnt2a standard conditions Prim-25 dorsal aorta lumenized, normal
Prim-25 endothelial cell amount, normal
Prim-25 posterior cardinal vein lumenized, normal
med14s231/s231; s843Tg standard conditions Prim-25 dorsal aorta collapsed, abnormal
Prim-25 endothelial cell amount, normal
Prim-25 posterior cardinal vein collapsed, abnormal
mtss233/s233; s843Tg standard conditions Prim-25 blood vessel morphogenesis disrupted, abnormal
Prim-25 dorsal aorta lumenized, normal
Prim-25 endothelial cell amount, normal
Prim-25 posterior cardinal vein duplicated, abnormal
Prim-25 posterior cardinal vein lumenized, normal
psns634/s634; s843Tg standard conditions Prim-25 blood vessel morphogenesis disrupted, abnormal
Prim-25 endothelial cell amount, normal
Prim-25 posterior cardinal vein increased diameter, abnormal
sestd1s828/s828; s843Tg standard conditions Prim-25 blood vessel morphogenesis disrupted, abnormal
Prim-25 dorsal aorta fused with posterior cardinal vein, abnormal
Prim-25 endothelial cell amount, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 307(1), Jin, S.W., Herzog, W., Santoro, M.M., Mitchell, T.S., Frantsve, J., Jungblut, B., Beis, D., Scott, I.C., D'Amico, L.A., Ober, E.A., Verkade, H., Field, H.A., Chi, N.C., Wehman, A.M., Baier, H., and Stainier, D.Y., A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos, 29-42, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.