ZFIN ID: ZDB-FIG-070801-14
Jin et al., 2007 - A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos. Developmental Biology   307(1):29-42 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 5 Mutations affecting vascular patterning. (A–T) Bright-field and epifluorescent micrographs of Tg(flk1:EGFP)s843unfs808 mutant embryos (D, E, 30 hpf; F, 48 hpf), slvs887 mutant (J–L, 78 hpf) and qads840 mutant (Q–R, 78 hpf) larvae and their wild-type (wt) siblings. (A–F) unfs808 mutants have shortened intersegmental vessels (SEs, arrow in panel F) and fail to form the dorsal longitudinal anastomotic vessel (DLAV, arrowhead in panels C and F) and appear to have reduced sprouting angiogenesis in the head (E). (G–L) Mis-patterning of the intersegmental vessels (SEs, arrow in panel L) and failure to remodel the posterior cardinal vein (PCV, arrowhead in L) in slvs887 mutant larvae. (M–S) Loop formation in the hindbrain capillary network in qads840 mutant larvae (P (wt) and T (qads840) are dorsal views focusing on the hindbrain capillary network). Instead of connecting lateral to medial as in wild-type (O, P), in qads840 mutant larvae capillaries connect back to their vessel of origin (arrowheads in panels S, T). Note that the tail vasculature in qads840 mutants (R) appears indistinguishable from wild-type (N). qads840 mutant embryos and their wild-type siblings were PTU treated to allow better visualization of the head vasculature.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
qads840/s840; s843Tg standard conditions Protruding-mouth blood vessel morphogenesis disrupted, abnormal
Protruding-mouth cranial vasculature misrouted, abnormal
Protruding-mouth post-vent vasculature spatial pattern, normal
slvs887/s887; s843Tg standard conditions Protruding-mouth blood vessel remodeling disrupted, abnormal
Protruding-mouth caudal vein plexus morphology, abnormal
Protruding-mouth intersegmental vessel position, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 307(1), Jin, S.W., Herzog, W., Santoro, M.M., Mitchell, T.S., Frantsve, J., Jungblut, B., Beis, D., Scott, I.C., D'Amico, L.A., Ober, E.A., Verkade, H., Field, H.A., Chi, N.C., Wehman, A.M., Baier, H., and Stainier, D.Y., A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos, 29-42, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.