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ZFIN ID: ZDB-FIG-070801-15
Jin et al., 2007 - A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos. Developmental Biology   307(1):29-42 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-15

Fig. 6 intersection regulates vascular patterning. (A–D) Lateral bright-field and epifluorescent micrographs of 32 hpf embryos. Vascular patterning defects can be observed by visualizing Tg(flk1:EGFP)s843 expression, but not via bright-field microscopy. ints413 mutants fail to connect the lateral dorsal aortae (LDA) to form the dorsal aorta (DA), and the anterior and posterior cardinal veins (ACV and PCV) to the common cardinal vein (CCV). (E–H) Dorsal views of ve-cadherin and Tg(flk1:EGFP)s843 expression. ints413 mutant embryos exhibit a pronounced dilation of the PCV (inset in panel D).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh5 TL standard conditions Prim-15 anterior cardinal vein ISH
Prim-15 common cardinal vein ISH
Prim-15 dorsal aorta ISH
Prim-15 lateral dorsal aorta ISH
Prim-15 posterior cardinal vein ISH
ints413/s413(TL) standard conditions Prim-15 anterior cardinal vein ISH
Prim-15 dorsal aorta ISH
Prim-15 lateral dorsal aorta ISH
Prim-15 posterior cardinal vein ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ints413/s413; s843Tg standard conditions Prim-15 anterior cardinal vein unfused from posterior cardinal vein, abnormal
Prim-15 blood vessel morphogenesis disrupted, abnormal
Prim-15 caudal vein plexus increased diameter, abnormal
Prim-15 common cardinal vein aplastic, abnormal
Prim-15 lateral dorsal aorta unfused from lateral dorsal aorta, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 307(1), Jin, S.W., Herzog, W., Santoro, M.M., Mitchell, T.S., Frantsve, J., Jungblut, B., Beis, D., Scott, I.C., D'Amico, L.A., Ober, E.A., Verkade, H., Field, H.A., Chi, N.C., Wehman, A.M., Baier, H., and Stainier, D.Y., A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos, 29-42, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.