ZFIN ID: ZDB-FIG-070613-66
Shin et al., 2007 - Bmp and Fgf signaling are essential for liver specification in zebrafish. Development (Cambridge, England)   134(11):2041-2050 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. 2 Alk8 is required for early liver development. Wild-type and alk8 mutant zebrafish embryos at 26 (A-H), 28 (I,J) and 48 (K,L) hpf were analyzed for hhex (A,B), prox1 (C,D), gata4 (E,F), gata6 (G,H), foxa3 (I,J) and cp (K,L) expression. (A,B) hhex expression in the liver region (arrows) is greatly reduced in the mutants, whereas its expression in the pancreatic islet appears unaffected (arrowheads). (C,D) prox1 expression in the liver region (arrows) is also greatly reduced in the mutants, whereas its expression in the interrenal primordium appears unaffected (arrowheads). To better visualize hepatic prox1 expression, a side-view image is shown in an inset. (E-H) gata4 and gata6 expression is reduced in the liver region (arrows), whereas their expression in the intestinal endoderm is only mildly affected (brackets). (I,J) The leftward bending of the gut appears unaffected in the mutants (brackets). (K,L) Hepatocyte expression of cp is completely absent in the mutants. All images, except insets, are dorsal views, anterior left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cp WT standard conditions Long-pec hepatocyte ISH
acvr1ltm110b/tm110b(TU) standard conditions Long-pec Not Detected hepatocyte ISH
foxa3 WT standard conditions Prim-5 gut ISH
acvr1ltm110b/tm110b(TU) standard conditions Prim-5 gut ISH
gata4 WT standard conditions Prim-5 intestinal epithelium ISH
Prim-5 liver primordium ISH
acvr1ltm110b/tm110b(TU) standard conditions Prim-5 intestinal epithelium ISH
Prim-5 liver primordium ISH
gata6 WT standard conditions Prim-5 intestinal epithelium ISH
Prim-5 liver primordium ISH
acvr1ltm110b/tm110b(TU) standard conditions Prim-5 intestinal epithelium ISH
Prim-5 liver primordium ISH
hhex WT standard conditions Prim-5 liver primordium ISH
Prim-5 pancreatic bud ISH
acvr1ltm110b/tm110b(TU) standard conditions Prim-5 liver primordium ISH
Prim-5 pancreatic bud ISH
prox1a WT standard conditions Prim-5 interrenal primordium ISH
Prim-5 liver primordium ISH
acvr1ltm110b/tm110b(TU) standard conditions Prim-5 interrenal primordium ISH
Prim-5 liver primordium ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development