Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-FIG-070613-68
Shin et al., 2007 - Bmp and Fgf signaling are essential for liver specification in zebrafish. Development (Cambridge, England)   134(11):2041-2050 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec

Fig. 4 Overexpression of wild-type alk8 under a heat-shock promoter rescued liver defects in alk8 mutant zebrafish embryos. (A-R) Embryos obtained from crossing alk8+/- females with Tg(hsp70:alk8);alk8+/- males were heat shocked at 18 (A,C,G,I,M,O) or 34 (D,F,J,L,P,R) hpf, and harvested at 34 (A-C,G-I), 42 (D-F,J-L), 47 (M-O) or 54 (P-R) hpf. The expression of hhex (A-F), prox1 (G-L) and cp (M-R) was then examined (arrows). When alk8 was overexpressed at 18 hpf, the expression of hhex, prox and cp in the mutant embryos (C,I,O, arrows) was comparable to that in wild-type siblings (A,G,M, arrows), whereas their expression in the mutant embryos that were not heat shocked was strongly reduced (B,H,N, arrows). Even when alk8 was overexpressed at 34 hpf, hhex, prox and cp expression was substantial in the mutant embryos (F,L,R, arrows) compared with the mutant embryos that were not head shocked (E,K,Q, arrows).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cp WT standard conditions High-pec hepatocyte ISH
Long-pec hepatocyte ISH
acvr1ltm110b/tm110b(TU) standard conditions High-pec Not Detected hepatocyte ISH
Long-pec Not Detected hepatocyte ISH
hhex WT standard conditions Prim-15 liver ISH
Prim-15 pancreatic bud ISH
Prim-25 liver ISH
Prim-25 pancreatic bud ISH
acvr1ltm110b/tm110b(TU) standard conditions Prim-15 liver ISH
Prim-15 pancreatic bud ISH
Prim-25 liver ISH
Prim-25 pancreatic bud ISH
prox1a WT standard conditions Prim-15 interrenal primordium ISH
Prim-15 liver ISH
Prim-25 interrenal primordium ISH
Prim-25 liver ISH
acvr1ltm110b/tm110b(TU) standard conditions Prim-15 interrenal primordium ISH
Prim-15 liver ISH
Prim-25 interrenal primordium ISH
Prim-25 liver ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development