Establishment of DV patterning is disturbed in MZspg. Embryos are shown in animal views, if not indicated otherwise. (A) bmp2b initiates at the sphere stage in the wild type. (A') bmp2b is absent in MZspg at sphere stage. (B) bmp7 initiates like bmp2b at sphere stage. (B') bmp7 is strongly reduced at its initiation in MZspg.(C) bmp4 is initiated slightly later than bmp2b and bmp7 within the wild type. (C') bmp4 fails to be initiated in MZspg. (D) vox is strongly expressed during blastula stages within the wild type and is absent from the dorsal-most region. (D') vox is lost in animal regions in MZspg; however, expression remains at the ventrolateral margin. (E) vent is similarly expressed as vox in the wild type during blastula stages. (E') vent is strongly reduced in MZspg; however, ventrolateral expression remains. (F,F') During gastrulation, eve1 expression is severely reduced in MZspg. Insets show ventral views. (G,G') bmp2, (H,H') bmp4 and (I,I') bmp7 fail to be expressed in MZspg. (G') bmp2 expression in the YSL is unchanged in MZspg. (J) nog1 is faintly initiated at the dorsal side in the wild type at sphere stage and similarly expressed at dome stage. (J') nog1 is strongly expressed in MZspg and expands ectopically to ventrolateral regions by dome stage. (K,K') chd initiates at sphere stage, similar to MZspg embryos. (L,M) chd is similarly expressed slightly later in the wild type. (L') In MZspg chd is nearly ubiquitously expressed shortly after initiation. (M') Ectopic expression persists until beginning of gastrulation. (N,O) gsc is expressed at the dorsal margin in the wild-type at blastula stages, and is dorsally confined when the shield forms. (N',O') gsc is expanded ventrally within the germ ring by 30-40% E, and is ectopically expressed within the entire germ ring by beginning of shield stage. (P,P') boz expression in the presumptive dorsal organizer is normal in MZspg embryos. (Q,Q') hhex expression in the dorso-marginal YSL is normal in MZspg.
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