FIGURE

Fig. 6

ID

ZDB-FIG-060706-26

Publication

Reim et al., 2006 - Maternal control of vertebrate dorsoventral axis formation and epiboly by the POU domain protein Spg/Pou2/Oct4

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Fig. 6

Spatial requirement for pou2. Animal views, if not noted otherwise. (A-M) An asterisk indicates the shield. (F',H,J,L,N) Upper half with and lower half without phase contrast to show the shield, or expression, respectively. (A'-M') Bright-field images of injected living embryos were merged with UV images taken with the same adjustment. (A''-M'') UV images show EGFP expression, corresponding to A'-M'. (A-J) pou2 mRNA injection into ventro-marginal blastomeres of MZspg embryos at early cleavage stages can restore bmp4 expression. (B') bmp4 expression at beginning of shield stage in the wild-type embryo. (K-N) pou2 mRNA injection into dorsal or animal blastomeres of MZspg embryos at early cleavage stages cannot restore bmp4 expression.

Expression Data

Gene:	bmp4
Fish:	WT
Anatomical Term:	unspecified
Stage Range:	50%-epiboly to Shield

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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