ZFIN ID: ZDB-FIG-060605-33
Hutchinson et al., 2006 - Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify motoneuron subtype identity. Development (Cambridge, England)   133(11):2137-2147 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 4 Islet1 is required to inhibit interneuron formation. (A,B) Embryos co-stained with Lhx3 (red) and zn1 (green) Abs. (A) In control embryos, zn1 and Lhx3 were co-expressed in motoneurons that projected axons (arrowheads) out of the spinal cord and in VeLD interneurons (one is indicated by an arrow; slanted lines denote somite boundaries). (B) islet1 MO-injected embryos had cells that co-expressed Lhx3 and zn1, but they did not project axons out of the spinal cord, and instead had axons that projected caudally within the spinal cord. (C,D) Embryos stained with GABA Ab. (C) In control embryos, GABA Ab reveals KA'', KA', VeLD and other (unidentified) interneurons; cells in the V-K position are marked by asterisks. (D) Cells in the V-K position (asterisks) are more numerous in islet1 MO-injected embryos. (E,F) The same embryos shown in C and D, but here showing co-labeling with GABA (red) and zn1 (green) Abs. Dots indicate cells co-expressing GABA and zn1. (E) In control embryos, only a few cells co-express these markers. Arrowheads in E indicate CaP axons. (F) In islet1 MO-injected embryos there are many more cells that co-express zn1 and GABA. All embryos shown in this figure are at 28 hpf. Scale bar: 20 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lhx3 AB standard conditions Prim-5 primary motor neuron IHC
Prim-5 VeLD IHC
AB + MO2-isl1a + MO3-isl1a standard conditions Prim-5 primary motor neuron IHC
Prim-5 VeLD IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-1 IHC AB standard conditions Prim-5 primary motor neuron
IHC Prim-5 VeLD
IHC AB + MO2-isl1a + MO3-isl1a standard conditions Prim-5 primary motor neuron
IHC Prim-5 VeLD
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-isl1a + MO3-isl1a standard conditions Prim-5 Kolmer-Agduhr neuron increased amount, abnormal
Prim-5 motor neuron axon mislocalised posteriorly, abnormal
Prim-5 VeLD increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development