ZFIN ID: ZDB-FIG-060605-34
Hutchinson et al., 2006 - Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify motoneuron subtype identity. Development (Cambridge, England)   133(11):2137-2147 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5

Fig. 5 Misexpression of islet1 RNA induces PMN formation and inhibits interneuron formation. (A,B) 28 hpf embryos labeled with zn1 and znp1 (green) and Islet (red) Abs. Control embryos (A) have MiP axons (arrows), CaP axons (arrowheads) and several PMN cell bodies (inset; zn1 Ab; dots). Misexpression of islet1 RNA (B) causes a thicker ventral motor nerve (arrowheads) and more zn1-positive cell bodies (inset, dots). (C,D) 20 hpf embryos labeled with islet2 riboprobe. islet2 is expressed in one or two PMNs per segment (black dots) in control embryos (C); the second cell is VaP, a duplicate CaP that is sometimes present and typically dies (Eisen et al., 1990). islet2 is expressed in two to four PMNs per segment in embryos misexpressing islet1 RNA (D). (E,F) 28 hpf embryos labeled with GABA Ab. The number of cells in the V-K position (asterisks) is decreased in embryos misexpressing islet1 RNA (F) when compared with controls (E). Scale bar: 20 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
isl2a AB standard conditions 20-25 somites primary motor neuron ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-isl IHC AB standard conditions Prim-5 primary motor neuron
zn-1 IHC AB standard conditions Prim-5 CaP motoneuron axon
IHC Prim-5 MiP motor neuron axon
IHC Prim-5 primary motor neuron
znp-1 IHC AB standard conditions Prim-5 CaP motoneuron axon
IHC Prim-5 MiP motor neuron axon
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development