ZFIN ID: ZDB-FIG-060605-35
Hutchinson et al., 2006 - Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify motoneuron subtype identity. Development (Cambridge, England)   133(11):2137-2147 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 6 Formation of CaP subtype identity is independent of Islet2. (A,B) Embryos labeled with zn1 and znp1 Abs (green). Arrows indicate MiP axons, arrowheads indicate CaP axons. At 28 hpf, control embryos (A) have both CaP and MiP axons. Embryos misexpressing islet2 RNA (B) also have normal MiP and CaP axons. (C,D) Embryos labeled with zn1 and znp1 (green), and Islet (red) Abs. Arrowheads indicate CaP axons. CaP cell bodies (dots) co-label with Islet and zn1 Abs and project axons ventrally at 28 hpf in control embryos (C). islet2 MO-injected embryos (D) lack Islet staining in CaP cell bodies and have abnormal CaP axons. Scale bar: 20 ┬Ám.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab2-isl IHC AB standard conditions Prim-5 CaP motoneuron
IHC AB + MO1-isl2a standard conditions Prim-5 Not Detected CaP motoneuron
zn-1 IHC AB standard conditions Prim-5 CaP motoneuron
IHC Prim-5 CaP motoneuron axon
IHC Prim-5 MiP motor neuron axon
IHC AB + MO1-isl2a standard conditions Prim-5 CaP motoneuron
IHC Prim-5 CaP motoneuron axon
IHC Prim-5 MiP motor neuron
znp-1 IHC AB standard conditions Prim-5 CaP motoneuron
IHC Prim-5 CaP motoneuron axon
IHC Prim-5 MiP motor neuron axon
IHC AB + MO1-isl2a standard conditions Prim-5 CaP motoneuron
IHC Prim-5 CaP motoneuron axon
IHC Prim-5 MiP motor neuron
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-isl2a standard conditions Prim-5 CaP motoneuron axon branchiness, abnormal
Prim-5 CaP motoneuron axon extension decreased process quality, abnormal
Prim-5 CaP motoneuron axon guidance physical quality of a process, abnormal
Prim-5 CaP motoneuron axon truncated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development