ZFIN ID: ZDB-FIG-060605-32
Hutchinson et al., 2006 - Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify motoneuron subtype identity. Development (Cambridge, England)   133(11):2137-2147 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 3 Islet proteins are required for SMN formation. (A-D) 72 hpf embryos stained with Neurolin (green) and Islet (red) Abs. For each panel, one segment (outlined) is magnified and shown below (A'-D'). Control embryos (A) had dorsally projecting (arrows) and ventrally projecting (arrowheads) Neurolin-positive SMNs and many Islet-positive cells. islet1 MO-injected embryos (B) lacked SMN axons and had 52% fewer Islet-positive cells (*P<0.01, 18 segments of three embryos). islet2 MO-injected embryos (C) had 10% fewer SMN cell bodies (*P<0.01, 24 segments of four embryos). These embryos entirely lacked dorsally projecting SMN axons and ventrally projecting SMN axons (arrowheads) were disorganized. The cells labeled with Islet Ab outside the neural tube next to the ventrally projecting axons are most likely dorsal root ganglion cells that are out of position. Embryos co-injected with islet1 and islet2 MOs (D) lacked all SMNs and 99.5% of Islet-positive cells were absent (*P<0.01, 18 segments of three embryos), although Neurolin-positive floor plate was still present. Scale bars: 20 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
alcama AB standard conditions Protruding-mouth floor plate IHC
Protruding-mouth secondary motor neuron IHC
AB + MO1-isl2a standard conditions Protruding-mouth floor plate IHC
Protruding-mouth secondary motor neuron IHC
AB + MO1-isl2a + MO2-isl1a + MO3-isl1a standard conditions Protruding-mouth floor plate IHC
Protruding-mouth secondary motor neuron IHC
AB + MO2-isl1a + MO3-isl1a standard conditions Protruding-mouth floor plate IHC
Protruding-mouth secondary motor neuron IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-isl IHC AB standard conditions Protruding-mouth primary motor neuron
IHC Protruding-mouth Rohon-Beard neuron
IHC AB + MO1-isl2a standard conditions Protruding-mouth primary motor neuron
IHC Protruding-mouth Rohon-Beard neuron
IHC AB + MO1-isl2a + MO2-isl1a + MO3-isl1a standard conditions Protruding-mouth primary motor neuron
IHC Protruding-mouth Rohon-Beard neuron
IHC AB + MO2-isl1a + MO3-isl1a standard conditions Protruding-mouth primary motor neuron
IHC Protruding-mouth Rohon-Beard neuron
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-isl2a standard conditions Protruding-mouth secondary motor neuron axon decreased amount, abnormal
Protruding-mouth secondary motor neuron decreased amount, abnormal
WT + MO1-isl2a + MO2-isl1a + MO3-isl1a standard conditions Protruding-mouth secondary motor neuron absent, abnormal
WT + MO2-isl1a + MO3-isl1a standard conditions Protruding-mouth secondary motor neuron axon decreased amount, abnormal
Protruding-mouth secondary motor neuron axon disorganized, abnormal
Protruding-mouth secondary motor neuron decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development