ZFIN ID: ZDB-FIG-060605-31
Hutchinson et al., 2006 - Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify motoneuron subtype identity. Development (Cambridge, England)   133(11):2137-2147 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 20-25 somites

Fig. 2 Islet1 is required for PMN formation. (A-C) 20 hpf embryos stained with islet2 riboprobe. Control embryos (A) express islet2 in RBs (dorsally located cells) and CaPs (ventrally located cells). islet1 MO-injected embryos (B) lack islet2 expression. islet1 MO-injected embryos co-injected with islet1 RNA (C) also lack most islet2 expression; one islet2-positive PMN is indicated by a black dot. (D-F) 28 hpf embryos stained with zn1 and znp1 Abs (green). Control embryos (D) have dorsally projecting MiP axons (arrows) and ventrally projecting CaP axons (arrowheads). islet1 MO-injected embryos (E) lack both MiP and CaP axons. Co-injection of islet1 MO and islet1 RNA (F) restored both MiP and CaP axons. Scale bar: 20 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
isl2a AB standard conditions 20-25 somites CaP motoneuron ISH
20-25 somites MiP motor neuron ISH
20-25 somites primary motor neuron ISH
20-25 somites Rohon-Beard neuron ISH
AB + MO2-isl1a + MO3-isl1a standard conditions 20-25 somites Not Detected CaP motoneuron axon ISH
20-25 somites Not Detected MiP motor neuron axon ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-1 IHC AB standard conditions 20-25 somites primary motor neuron
IHC AB + MO2-isl1a + MO3-isl1a standard conditions 20-25 somites primary motor neuron
znp-1 IHC AB standard conditions 20-25 somites primary motor neuron
IHC AB + MO2-isl1a + MO3-isl1a standard conditions 20-25 somites primary motor neuron
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-isl1a + MO3-isl1a standard conditions 20-25 somites CaP motoneuron axon absent, abnormal
20-25 somites MiP motor neuron axon absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development