ZFIN ID: ZDB-FIG-060125-7
Croushore et al., 2005 - Ptena and ptenb genes play distinct roles in zebrafish embryogenesis. Developmental dynamics : an official publication of the American Association of Anatomists   234(4):911-921 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Day 4

Fig. 6 Effect of ptena-MO1 morpholino on zebrafish inner ear development. All panels are lateral views of the otic vesicle with anterior to the left. A: Twenty-four hpf, wild type embryo. B-D: Twenty-four hpf, ptena morphants. E: Forty-eight hpf, wild type embryo. F: Forty-eight hpf, ptena morphant. G: Four dpf, wild type embryo. H-J: Four dpf, ptena morphants. K-T: Forty-eight hpf, in situ hybridization using ear-specific markers. K: pax2a, wild type embryo. L: pax2a, ptena morphant. M: otx1, wild type embryo. N: otx1, ptena morphant. O: starmaker, wild type embryo. P: starmaker, ptena morphant. Q: dfna5, wild type embryo. R: dfna5, ptena morphant. S: ncs-1a, wild type embryo. T: ncs1-a, ptena morphant. Scale bars in A-J = 25 μm. Arrowhead indicates site where epithelial pillars fail to fuse. Arrows point to abnormal tissue masses.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gsdmeb WT standard conditions Long-pec semicircular canal ISH
WT + MO1-ptena standard conditions Long-pec semicircular canal ISH
ncs1a WT standard conditions Long-pec semicircular canal ISH
WT + MO1-ptena standard conditions Long-pec semicircular canal ISH
otx1 WT standard conditions Long-pec otic vesicle ISH
WT + MO1-ptena standard conditions Long-pec otic vesicle ISH
pax2a WT standard conditions Long-pec otic vesicle ISH
WT + MO1-ptena standard conditions Long-pec otic vesicle ISH
stm WT standard conditions Long-pec otic vesicle ISH
WT + MO1-ptena standard conditions Long-pec otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ptena standard conditions Prim-5 otolith decreased amount, abnormal
Prim-5 otolith shape, abnormal
Long-pec otolith decreased amount, abnormal
Day 4 inner ear epithelial cell aggregated, abnormal
Day 4 inner ear epithelial cell disorganized, abnormal
Day 4 otolith decreased amount, abnormal
Day 4 pillar of the semicircular canal unfused from pillar of the semicircular canal, abnormal
Day 4 semicircular canal formation disrupted, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.