ZFIN ID: ZDB-FIG-060125-9
Croushore et al., 2005 - Ptena and ptenb genes play distinct roles in zebrafish embryogenesis. Developmental dynamics : an official publication of the American Association of Anatomists   234(4):911-921 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 8 Lipid phosphatase activity of zebrafish pten genes. Lysates were prepared from wild type embryos as well as ptena and ptenb morphants at 48 hpf (30 embryos/group). Proteins were separated by SDS-PAGE and transferred to a nitrocellulose filter. Blots were probed with an anti-Akt antibody, stripped, and reprobed with an anti-phospho-Akt (pAkt) antibody. A: Western blot. B: Quantitation of bands by laser densitometry. The bars for ptena (n = 6 separate experiments) and ptenb (n = 4 separate experiments) represent the relative ratio of pAkt to total Akt (tAkt) compared to wild type embryos. The error bars represent the standard error of the mean. The asterisk indicates a statistically significant increase in the ratio of pAkt to tAkt (P < 0.05) as calculated by an unpaired Students t-test.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ptena standard conditions Long-pec lipid phosphatase activity disrupted, abnormal
Long-pec phosphatidylinositol dephosphorylation decreased occurrence, abnormal
WT + MO1-ptenb standard conditions Long-pec lipid phosphatase activity disrupted, abnormal
Long-pec phosphatidylinositol dephosphorylation decreased occurrence, abnormal
Acknowledgments:
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