Fig. 3
- ID
- ZDB-FIG-051013-6
- Publication
- Mavropoulos et al., 2005 - sox4b is a key player of pancreatic alpha cell differentiation in zebrafish
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Expression of sox4b in pancreas observed by FISH. (A–L) Expression of either pdx-1, neuroD, nkx2.2, isl1 (left column), or sox4b (central column) in 20 hpf embryos. The right column presents the overlay of the respective left and middle panels. (M, N) Overlay of sox4b- and either insulin- (M) or somatostatin2 (N) expression in 20 hpf embryos. (O, P) Expression of glucagon (O) and sox4b (P) in 36 hpf embryos. (Q) Overlay of panels O and P. (Q) Overlay of sox4b- and insulin expression in 26 hpf embryos. All the panels are optical sections of ventrally viewed yolk-free embryos (A–Q) and laterally viewed yolk-free embryo (R), with anterior to the left. sox4b was detected with a digoxigenin-labeled anti-sense probe and a tyramide-Cy3 substrate and the other markers with DNP-labeled anti-sense probes and tyramide-FITC substrate (see Materials and methods). A–N scale bars = 50 μm; O–Q scale bars = 25 μm. The arrowheads point to cells where sox4b is co-expressed with another marker, arrows in panels P and Q point to cells expressing only sox4b. |
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| Stage Range: | 20-25 somites to Prim-25 |
Reprinted from Developmental Biology, 285(1), Mavropoulos, A., Devos, N., Biemar, F., Zecchin, E., Argenton, F., Edlund, H., Motte, P., Martial, J.A., and Peers, B., sox4b is a key player of pancreatic alpha cell differentiation in zebrafish, 211-223, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.