sox4b is a key player of pancreatic alpha cell differentiation in zebrafish

Mavropoulos, A., Devos, N., Biemar, F., Zecchin, E., Argenton, F., Edlund, H., Motte, P., Martial, J.A., and Peers, B.
Developmental Biology   285(1): 211-223 (Journal)
Registered Authors
Argenton, Francesco, Biemar, Frédéric, Devos, Nathalie, Martial, Joseph A., Mavropoulos, Anastasia, Peers, Bernard, Zecchin, Elisabetta
SOX, Transcription factor, Pancreas, Zebrafish, Development, Glucagon
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Differentiation
  • DNA/genetics
  • Gene Expression Regulation, Developmental
  • Glucagon/metabolism
  • High Mobility Group Proteins/genetics
  • High Mobility Group Proteins/physiology*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Islets of Langerhans/cytology*
  • Islets of Langerhans/embryology
  • Islets of Langerhans/physiology*
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Antisense/administration & dosage
  • RNA, Antisense/genetics
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Trans-Activators/genetics
  • Trans-Activators/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
16055112 Full text @ Dev. Biol.
Pancreas development relies on a network of transcription factors belonging mainly to the Homeodomain and basic Helix-Loop-Helix families. We show in this study that, in zebrafish, sox4, a member of the SRY-like HMG-box (SOX) family, is required for proper endocrine cell differentiation. We found that two genes orthologous to mammalian Sox4 are present in zebrafish and that only one of them, sox4b, is strongly expressed in the pancreatic anlage. Transcripts of sox4b were detected in mid-trunk endoderm from the 5-somite stage, well before the onset of expression of the early pancreatic gene pdx-1. Furthermore, by fluorescent double in situ hybridization, we found that expression of sox4b is mostly restricted to precursors of the endocrine compartment. This expression is not maintained in differentiated cells although transient expression can be detected in alpha cells and some beta cells. That sox4b-expressing cells belong to the endocrine lineage is further illustrated by their absence from the pancreata of slow-muscle-omitted mutant embryos, which specifically lack all early endocrine markers while retaining expression of exocrine markers. The involvement of sox4b in cell differentiation is suggested firstly by its up-regulation in mind bomb mutant embryos displaying accelerated pancreatic cell differentiation. In addition, sox4b knock-down leads to a drastic reduction in glucagon expression, while other pancreatic markers including insulin, somatostatin, and trypsin are not significantly affected. This disruption of alpha cell differentiation is due to down-regulation of the homeobox arx gene specifically in the pancreas. Taken together, these data demonstrate that, in zebrafish, sox4b is expressed transiently during endocrine cell differentiation and plays a crucial role in the generation of alpha endocrine cells.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes