FIGURE

Fig. 6

ID

ZDB-FIG-050722-4

Publication

Clements et al., 2005 - LZIC regulates neuronal survival during zebrafish development

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Fig. 6

Location of morphant tissue death. Whole mount in situs performed on embryos using a probe cocktail (cktl; A) containing ctsl (anterior ectoderm), opl (forebrain), en2 (midbrain and midbrain/hindbrain boundary), krox20 (rhombomeres three and five), and myoD (somites); pax6 (B); neurogenin (C); shh (D); and sna2 (E). Embryos in columns 2–4 are representative of increasingly severe phenotypes after injection with 3 ng LZIC MO1 at the 1–2 cell stage. Embryos in column 1 are either uninjected or injected with LZIC mismatch control morpholino (LZICmis MO). The CNS is most sensitive to loss of LZIC in the midbrain and spinal column. Telencephalon (red arrows), eye (purple arrows), diencephalon (yellow arrows), midbrain/hindbrain boundary (green arrows), hindbrain (black arrows), dorsal neural tube (blue arrows), somites (turquoise arrows). Lateral views, anterior to left.

Expression Data

Genes:	ctslb egr2b en2a myod1 neurog1 pax6a shha snai1b zic1
Fish:	WIK/AB WIK/AB + MO1-lzic
Knockdown Reagent:	MO1-lzic
Anatomical Terms:	diencephalon ectoderm floor plate forebrain head mesenchyme hindbrain immature eye integument midbrain hindbrain boundary neural crest neural rod neural tube notochord rhombomere 3 rhombomere 5 somite
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WIK/AB + MO1-lzic
Knockdown Reagent:	MO1-lzic
Observed In:	brain neural crest neural tube dorsal region
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 283(2), Clements, W.K., and Kimelman, D., LZIC regulates neuronal survival during zebrafish development, 322-334, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.