FIGURE

Fig. 3

ID

ZDB-FIG-050721-8

Publication

Qian et al., 2005 - Microarray analysis of zebrafish cloche mutant using amplified cDNA and identification of potential downstream target genes

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Fig. 3

Verification of target clones by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). One microgram of total RNA sample (20 embryos each) derived from the wild-type sibling (lanes 1 and 3) and clo mutant embryos (lanes 2 and 4) were reverse transcribed and subjected to PCR analysis with specific primers corresponding to each of the perspective target genes. PCR products were electrophoresed on 1% agarose gel and stained with ethidium bromide. e1-globin and elf1a were used as internal controls.

Expression Data

Genes:	cahz cldng cpox dusp5 efna2a hbbe1.1 mmp13a.1 ncf1 nrros rhogb tmem254 wu:fe14d06 yrk znfl2a
Fish:	WT npas4l^s5
Anatomical Term:	unspecified
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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