ZFIN ID: ZDB-FIG-050721-8
Qian et al., 2005 - Microarray analysis of zebrafish cloche mutant using amplified cDNA and identification of potential downstream target genes. Developmental dynamics : an official publication of the American Association of Anatomists   233(3):1163-1172 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: 14-19 somites

Fig. 3 Verification of target clones by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). One microgram of total RNA sample (20 embryos each) derived from the wild-type sibling (lanes 1 and 3) and clo mutant embryos (lanes 2 and 4) were reverse transcribed and subjected to PCR analysis with specific primers corresponding to each of the perspective target genes. PCR products were electrophoresed on 1% agarose gel and stained with ethidium bromide. e1-globin and elf1a were used as internal controls.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cahz WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites unspecified RTPCR
cldng WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites Not Detected whole organism RTPCR
cpox WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites unspecified RTPCR
dusp5 WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites unspecified RTPCR
efna2a WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites unspecified RTPCR
hbbe1.1 WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites unspecified RTPCR
mmp13a WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites Not Detected whole organism RTPCR
ncf1 WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites Not Detected whole organism RTPCR
nrros WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites Not Detected whole organism RTPCR
rhogb WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites Not Detected whole organism RTPCR
tmem254 WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites Not Detected whole organism RTPCR
wu:fe14d06 WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites unspecified RTPCR
yrk WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites unspecified RTPCR
znfl2a WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites unspecified RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.