Fig. 3 Verification of target clones by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). One microgram of total RNA sample (20 embryos each) derived from the wild-type sibling (lanes 1 and 3) and clo mutant embryos (lanes 2 and 4) were reverse transcribed and subjected to PCR analysis with specific primers corresponding to each of the perspective target genes. PCR products were electrophoresed on 1% agarose gel and stained with ethidium bromide. e1-globin and elf1a were used as internal controls.