ZFIN ID: ZDB-FIG-050721-6
Qian et al., 2005 - Microarray analysis of zebrafish cloche mutant using amplified cDNA and identification of potential downstream target genes. Developmental dynamics : an official publication of the American Association of Anatomists   233(3):1163-1172 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: 14-19 somites

Fig. 1 Genotyping of the 18-somite clo homozygous and wild-type/heterozygous sibling embryos by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Equal amount (1/10) of cDNA from the randomly selected single 18-somite embryo was subjected to semiquantitative PCR analysis with specific primers for zebrafish flk1, scl, lmo2, gata1, and e1-globin. actin and elf1a were used as control to indicate the amount of cDNA used. Lanes 4, 7, and 9 are clo mutants, whereas the other lanes (lanes 1, 2, 3, 5, 6, 8, 10, 11, and 12) represent wild-type or heterozygous sibling embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
efna2a WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites unspecified RTPCR
gata1a WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites Not Detected whole organism RTPCR
hbbe1.1 WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites Not Detected whole organism RTPCR
kdrl WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites Not Detected whole organism RTPCR
lmo2 WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites Not Detected whole organism RTPCR
tal1 WT standard conditions 14-19 somites unspecified RTPCR
npas4ls5 standard conditions 14-19 somites Not Detected whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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