ZFIN ID: ZDB-FIG-050721-6 |
Qian et al., 2005
- Microarray analysis of zebrafish cloche mutant using amplified cDNA and identification of potential downstream target genes.
Developmental dynamics : an official publication of the American Association of Anatomists
233(3):1163-1172
Full text @ Dev. Dyn.
EXPRESSION / LABELING:
Genes: | |
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Fish: | |
Anatomical Term: | |
Stage: | 14-19 somites |
Fig. 1 Genotyping of the 18-somite clo homozygous and wild-type/heterozygous sibling embryos by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Equal amount (1/10) of cDNA from the randomly selected single 18-somite embryo was subjected to semiquantitative PCR analysis with specific primers for zebrafish flk1, scl, lmo2, gata1, and e1-globin. actin and elf1a were used as control to indicate the amount of cDNA used. Lanes 4, 7, and 9 are clo mutants, whereas the other lanes (lanes 1, 2, 3, 5, 6, 8, 10, 11, and 12) represent wild-type or heterozygous sibling embryos. |
Gene Expression Details
Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
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efna2a | WT | standard conditions | 14-19 somites | unspecified | RTPCR | ||
npas4ls5 | standard conditions | 14-19 somites | unspecified | RTPCR | |||
gata1a | WT | standard conditions | 14-19 somites | unspecified | RTPCR | ||
npas4ls5 | standard conditions | 14-19 somites | Not Detected | whole organism | RTPCR | ||
hbbe1.1 | WT | standard conditions | 14-19 somites | unspecified | RTPCR | ||
npas4ls5 | standard conditions | 14-19 somites | Not Detected | whole organism | RTPCR | ||
kdrl | WT | standard conditions | 14-19 somites | unspecified | RTPCR | ||
npas4ls5 | standard conditions | 14-19 somites | Not Detected | whole organism | RTPCR | ||
lmo2 | WT | standard conditions | 14-19 somites | unspecified | RTPCR | ||
npas4ls5 | standard conditions | 14-19 somites | Not Detected | whole organism | RTPCR | ||
tal1 | WT | standard conditions | 14-19 somites | unspecified | RTPCR | ||
npas4ls5 | standard conditions | 14-19 somites | Not Detected | whole organism | RTPCR |
Antibody Labeling Details
No data available
Phenotype Details
No data available
Acknowledgments: | This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn. |
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