ZFIN Figure: Qian et al., 2005, Fig. 1

ZFIN ID: ZDB-FIG-050721-6

ZFIN ID: ZDB-FIG-050721-6

Qian et al., 2005 - Microarray analysis of zebrafish cloche mutant using amplified cDNA and identification of potential downstream target genes. Developmental dynamics : an official publication of the American Association of Anatomists 233(3):1163-1172 Full text @ Dev. Dyn.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	efna2a gata1a hbbe1.1 kdrl lmo2 tal1
Fish:	WT npas4l^s5
Anatomical Term:	unspecified
Stage:	14-19 somites

Fig. 1 Genotyping of the 18-somite clo homozygous and wild-type/heterozygous sibling embryos by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Equal amount (1/10) of cDNA from the randomly selected single 18-somite embryo was subjected to semiquantitative PCR analysis with specific primers for zebrafish flk1, scl, lmo2, gata1, and e1-globin. actin and elf1a were used as control to indicate the amount of cDNA used. Lanes 4, 7, and 9 are clo mutants, whereas the other lanes (lanes 1, 2, 3, 5, 6, 8, 10, 11, and 12) represent wild-type or heterozygous sibling embryos.

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
efna2a	WT	standard conditions	14-19 somites		unspecified	RTPCR
	npas4l^s5	standard conditions	14-19 somites		unspecified	RTPCR
gata1a	WT	standard conditions	14-19 somites		unspecified	RTPCR
	npas4l^s5	standard conditions	14-19 somites	Not Detected	whole organism	RTPCR
hbbe1.1	WT	standard conditions	14-19 somites		unspecified	RTPCR
	npas4l^s5	standard conditions	14-19 somites	Not Detected	whole organism	RTPCR
kdrl	WT	standard conditions	14-19 somites		unspecified	RTPCR
	npas4l^s5	standard conditions	14-19 somites	Not Detected	whole organism	RTPCR
lmo2	WT	standard conditions	14-19 somites		unspecified	RTPCR
	npas4l^s5	standard conditions	14-19 somites	Not Detected	whole organism	RTPCR
tal1	WT	standard conditions	14-19 somites		unspecified	RTPCR
	npas4l^s5	standard conditions	14-19 somites	Not Detected	whole organism	RTPCR

Antibody Labeling Details No data available

Phenotype Details No data available

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.