ZFIN ID: ZDB-PERS-971016-20
Trede, Nick
Email: nikolaus.trede@hci.utah.edu
URL: http://www.huntsmancancer.org/research/labs/trede-lab
Affiliation: Trede Lab
Address: Huntsman Cancer Insitute Suite 4265 2000, Circle of Hope Salt Lake City, UT 84112 USA
Country: United States
Phone: 801-585-0199 (O) 801-587-3054 (L)
Fax: 801-585-5470


A. Baccalaureate Degree: not awarded in German system. Finished pre-med studies at Albert Ludwigs University Medical School, Freiburg in 7/1982

B. Advanced Degrees: 12/1987, Albert Ludwigs University Medical School, Freiburg, MD; 12/1992, Université Paris VII, PhD in immunology

C. Pediatric internship, 7/1993-6/1994, Children's Hospital, Boston
Junior Assistant Resident in Pediatrics, 7/1994-6/1995, Children's Hospital, Boston
Clinical Fellow in Pediatric Hematology/Oncology, 7/1995-6/1998, Children's Hospital/DFCI, Boston

D. Specialty and Sub-specialty Board Certifications:
Pediatrics: 1998
Pediatric Hematology/Oncology: 2000

E. Postdoctoral (Ph.D.) Fellowships: through 6/2004, Children's Hospital,
Boston, laboratory of Len Zon, funded through K08


7/1998-6/2004: Pediatric Hematology/Oncology
Attending Physician for inpatient and outpatient services at Children's Hospital and DFCI, Boston
Instructor in Pediatrics, Harvard Medical School

Since 7/2004: Assistant Professor in Pediatrics, University of Utah
Adjunct Assistant Professor in Oncologic Sciences


My lab is interested in modeling human diseases of the immune system in zebrafish. These diseases include immunodeficiency (such as DiGeorge syndrome and SCID), autoimmunity and leukemia.
We are also involved in exploring the innate immune system using a transgenic approach in zebrafish.

Demy, D.L., Tauzin, M., Lancino, M., le Cabec, V., Redd, M., Murayama, E., Maridonneau-Parini, I., Trede, N., Herbomel, P. (2017) Trim33 / Tif1-γ is essential for macrophage and neutrophil mobilisation to developmental or inflammatory cues. Journal of Cell Science. 130(17):2797-2807
Murayama, E., Sarris, M., Redd, M., Le Guyader, D., Vivier, C., Horsley, W., Trede, N., Herbomel, P. (2015) NACA deficiency reveals the crucial role of somite-derived stromal cells in haematopoietic niche formation. Nature communications. 6:8375
Trede, N.S., Heaton, W., Ridges, S., Sofla, H., Cusick, M., Bearss, D., Jones, D., and Fujinami, R.S. (2013) Discovery of Biologically Active Oncologic and Immunologic Small Molecule Therapies using Zebrafish: Overview and Example of Modulation of T Cell Activation. Current Protocols in Pharmacology. 14:4.24
Choudhry, P., and Trede, N.S. (2013) DiGeorge Syndrome Gene tbx1 Functions through wnt11r to Regulate Heart Looping and Differentiation. PLoS One. 8(3):e58145
Blackburn, J.S., Liu, S., Raiser, D.M., Martinez, S.A., Feng, H., Meeker, N.D., Gentry, J., Neuberg, D., Look, A.T., Ramaswamy, S., Bernards, A., Trede, N.S., and Langenau, D.M. (2012) Notch signaling expands a pre-malignant pool of T-cell acute lymphoblastic leukemia clones without affecting leukemia-propagating cell frequency. Leukemia. 26(9):2069-2078
Hutchinson, S.A., Tooke-Locke, E., Wang, J., Tsai, S., Katz, T., and Trede, N.S. (2012) Tbl3 regulates cell cycle length during zebrafish development. Developmental Biology. 368(2):261-272
Ridges, S., Heaton, W.L., Joshi, D., Choi, H., Eiring, A., Batchelor, L., Choudhry, P., Manos, E.J, Sofla, H., Sanati, A., Welborn, S., Agarwal, A., Spangrude, G.J., Miles, R.R., Cox, J.E., Frazer, J.K., Deininger, M., Balan, K., Sigman, M., Müschen, M., Perova, T., Johnson, R., Montpellier, B., Guidos, C.J., Jones, D.A., and Trede, N.S. (2012) Zebrafish screen identifies novel compound with selective toxicity against leukemia. Blood. 119(24):5621-5631
Cusick, M.F., Libbey, J.E., Trede, N.S., Eckels, D.D., and Fujinami, R.S. (2012) Human T cell expansion and experimental autoimmune encephalomyelitis inhibited by Lenaldekar, a small molecule discovered in a zebrafish screen. Journal of neuroimmunology. 244(1-2):35-44
Frazer, J.K., Batchelor, L.A., Bradley, D.F., Brown, K.H., Dobrinski, K.P., Lee, C., and Trede, N.S. (2012) Genomic Amplification of an Endogenous Retrovirus in Zebrafish T-Cell Malignancies. Advances in hematology. 2012:627920
Renshaw, S.A., and Trede, N.S. (2012) A model 450 million years in the making: zebrafish and vertebrate immunity. Disease models & mechanisms. 5(1):38-47
Rudner, L.A., Brown, K.H., Dobrinski, K.P., Bradley, D.F., Garcia, M.I., Smith, A.C., Downie, J.M., Meeker, N.D., Look, A.T., Downing, J.R., Gutierrez, A., Mullighan, C.G., Schiffman, J.D., Lee, C., Trede, N.S., and Frazer, J.K. (2011) Shared acquired genomic changes in zebrafish and human T-ALL. Oncogene. 30(41):4289-96
Choudhry, P., Joshi, D., Funke, B., and Trede, N. (2011) Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis. Developmental Biology. 349(2):483-493
Demarest, B.L., Horsely, W.H., Locke, E.E., Boucher, K., Grunwald, D.J., and Trede, N.S. (2011) Trans-centromere Effects on Meiotic Recombination in the Zebrafish. Genetics. 187(1):333-336
Mione, M.C., and Trede, N.S. (2010) The zebrafish as a model for cancer. Disease models & mechanisms. 3(9-10):517-523
Meeker, N.D., Smith, A.C., Frazer, J.K., Bradley, D.F., Rudner, L.A., Love, C., and Trede, N.S. (2010) Characterization of the zebrafish T cell receptor beta locus. Immunogenetics. 62(1):23-29
Mione, M., Meijer, A.H., Snaar-Jagalska, B.E., Spaink, H.P., and Trede, N.S. (2009) Disease modeling in zebrafish: cancer and immune responses-a report on a workshop held in Spoleto, Italy, July 20-22, 2009. Zebrafish. 6(4):445-451
Amigo, J.D., Ackermann, G.E., Cope, J.J., Yu, M., Cooney, J.D., Ma, D., Langer, N.B., Shafizadeh, E., Shaw, G.C., Horsley, W., Trede, N.S., Davidson, A.J., Barut, B.A., Zhou, Y., Wojiski, S.A., Traver, D., Moran, T.B., Kourkoulis, G., Hsu, K., Kanki, J.P., Shah, D.I., Lin, H.F., Handin, R.I., Cantor, A.B., and Paw, B.H. (2009) The role and regulation of friend of GATA-1 (FOG-1) during blood development in the zebrafish. Blood. 114(21):4654-4663
Frazer, J.K., Meeker, N.D., Rudner, L., Bradley, D.F., Smith, A.C., Demarest, B., Joshi, D., Locke, E.E., Hutchinson, S.A., Tripp, S., Perkins, S.L., and Trede, N.S. (2009) Heritable T-cell malignancy models established in a zebrafish phenotypic screen. Leukemia. 23(10):1825-1835
Lieschke, G.J., and Trede, N.S. (2009) Fish immunology. Current biology : CB. 19(16):R678-R682
Schwarz, K., Iolascon, A., Verissimo, F., Trede, N.S., Horsley, W., Chen, W., Paw, B.H., Hopfner, K.P., Holzmann, K., Russo, R., Esposito, M.R., Spano, D., De Falco, L., Heinrich, K., Joggerst, B., Rojewski, M.T., Perrotta, S., Denecke, J., Pannicke, U., Delaunay, J., Pepperkok, R., and Heimpel, H. (2009) Mutations affecting the secretory COPII coat component SEC23B cause congenital dyserythropoietic anemia type II. Nature Genetics. 41(8):936-940
Jima, D.D., Shah, R.N., Orcutt, T.M., Joshi, D., Law, J.M., Litman, G.W., Trede, N.S., and Yoder, J.A. (2009) Enhanced transcription of complement and coagulation genes in the absence of adaptive immunity. Molecular immunology. 46(7):1505-1516
Rudner, L.A., Frazer, J.K., and Trede, N.S. (2009) Modeling human hematologic malignancies in zebrafish: A review. Current Trends in Immunology. 10:119-124
Trede, N.S., Ota, T., Kawasaki, H., Paw, B.H., Katz, T., Demarest, B., Hutchinson, S., Zhou, Y., Hersey, C., Zapata, A., Amemiya, C.T., and Zon, L.I. (2008) Zebrafish mutants with disrupted early T-cell and thymus development identified in early pressure screen. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(9):2575-2584
Saltis, M., Criscitiello, M.F., Ohta, Y., Keefe, M., Trede, N.S., Goitsuka, R., and Flajnik, M.F. (2008) Evolutionarily conserved and divergent regions of the Autoimmune Regulator (Aire) gene: a comparative analysis. Immunogenetics. 60(2):105-114
Meeker, N.D., and Trede, N.S. (2008) Immunology and zebrafish: Spawning new models of human disease. Developmental and comparative immunology. 32(7):745-757
Aleström, P., Begemann, G., Carvan, M.J. 3rd, Cheng, K.C., Crosier, K., Crosier, P., Ekker, S., Huttenlocher, A., Kawakami, K., Kelly, G., Korzh, V., Lieschke, G., Mione, M., Neely, M.N., Neuhauss, S., and Trede, N.S. (2008) Views on four key questions about zebrafish research. Zebrafish. 5(1):9-24
Meeker, N.D., Hutchinson, S.A., Ho, L., and Trede, N.S. (2007) Method for isolation of PCR-ready genomic DNA from zebrafish tissues. Biotechniques. 43(5):610-614
Trede, N.S., Medenbach, J., Damianov, A., Hung, L.H., Weber, G.J., Paw, B.H., Zhou, Y., Hersey, C., Zapata, A., Keefe, M., Barut, B.A., Stuart, A.B., Katz, T., Amemiya, C.T., Zon, L.I., and Bindereif, A. (2007) Network of coregulated spliceosome components revealed by zebrafish mutant in recycling factor p110. Proceedings of the National Academy of Sciences of the United States of America. 104(16):6608-6613
Shaw, G.C., Cope, J.J., Li, L., Corson, K., Hersey, C., Ackermann, G.E., Gwynn, B., Lambert, A.J., Wingert, R.A., Traver, D., Trede, N.S., Barut, B.A., Zhou, Y., Minet, E., Donovan, A., Brownlie, A., Balzan, R., Weiss, M.J., Peters, L.L., Kaplan, J., Zon, L.I., and Paw, B.H. (2006) Mitoferrin is essential for erythroid iron assimilation. Nature. 440(7080):96-100
Yoder, J.A., Litman, R.T., Mueller, M.G., Desai, S., Dobrinski, K.P., Montgomery, J.S., Buzzeo, M.P., Ota, T., Amemiya, C.T., Trede, N.S., Wei, S., Djeu, J.Y., Humphray, S., Jekosch, K., Hernandez Prada, J.A., Ostrov, D.A., and Litman, G.W. (2004) Resolution of the novel immune-type receptor gene cluster in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 101(44):15706-15711
Ransom, D.G., Bahary, N., Niss, K., Traver, D., Burns, C., Trede, N.S., Paffett-Lugassy, N., Saganic, W.J., Lim, C.A., Hersey, C., Zhou, Y., Barut, B.A., Lin, S., Kingsley, P.D., Palis, J., Orkin, S.H., and Zon, L.I. (2004) The Zebrafish moonshine Gene Encodes Transcriptional Intermediary Factor 1gamma, an Essential Regulator of Hematopoiesis. PLoS Biology. 2(8):E237
Langenau, D.M., Ferrando, A.A., Traver, D., Kutok, J.L., Hezel, J.P., Kanki, J.P., Zon, L.I., Look, A.T., and Trede, N.S. (2004) In vivo tracking of T cell development, ablation, and engraftment in transgenic zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 101(19):7369-7374
Altmann, S.M., Mellon, M.T., Johnson, M.C., Paw, B.H., Trede, N.S., Zon, L.I., and Kim, C.H. (2004) Cloning and characterization of an Mx gene and its corresponding promoter from the zebrafish, Danio rerio. Developmental and comparative immunology. 28(4):295-306
Trede, N.S., Langenau, D.M., Traver, D., Look, A.T., and Zon, L.I. (2004) The use of zebrafish to understand immunity. Immunity. 20(4):367-379
Bahary, N., Davidson, A., Ransom, D., Shepard, J., Stern, H., Trede, N., Zhou, Y., Barut, B., and Zon, L.I. (2004) The Zon laboratory guide to positional cloning in zebrafish (2004). The Zebrafish: Genetics, Genomics and Informatics, 2nd ed., Methods Cell Biol.. 77:305-329
Paw, B.H., Davidson, A.J., Zhou, Y., Li, R., Pratt, S.J., Lee, C., Trede, N.S., Brownlie, A., Donovan, A., Liao, E.C., Ziai, J.M., Drejer, A.H., Guo, W., Kim, C.H., Gwynn, B., Peters, L.L., Chernova, M.N., Alper, S.L., Zapata, A., Wickramasinghe, S.N., Lee, M.J., Lux, S.E., Fritz, A., Postlethwait, J.H., and Zon, L.I. (2003) Cell-specific mitotic defect and dyserythropoiesis associated with erythroid band 3 deficiency. Nature Genetics. 34(1):59-64
Langenau, D.M., Traver, D., Ferrando, A.A., Kutok, J.L., Aster, J.C., Kanki, J.P., Lin, S., Prochownik, E., Trede, N.S., Zon, L.I., and Look, A.T. (2003) Myc-induced T cell leukemia in transgenic zebrafish. Science (New York, N.Y.). 299(5608):887-890
Traver, D., Herbomel, P., Patton, E.E., Murphey, R.D., Yoder, J.A., Litman, G.W., Catic, A., Amemiya, C.T., Zon, L.I., and Trede, N.S. (2003) The zebrafish as a model organism to study development of the immune system. Advances in immunology. 81:253-330
Liao, E.C., Trede, N.S., Ransom, D., Zapata, A., Kieran, M., and Zon, L.I. (2002) Non-cell autonomous requirement for the bloodless gene in primitive hematopoiesis of zebrafish. Development (Cambridge, England). 129(3):649-659
Trede, N.S., Zapata, A., and Zon, L.I. (2001) Fishing for lymphoid genes. Trends in immunology. 22(6):302-307
Trede, N.S., Ota, T., Kawasaki, H., Barut, B., Paw, B.H., Zhou, Y., Hersey, C., Zapata, A., Amemiya, C.T., and Zon, L.I. (2000) A genetic screen in the zebrafish defines mutations required for the development of the immune system. Blood. 96(11):2633
Paw, B.H., Zhou, Y., Li, R., Pratt, S.J., Davidson, A.J., Trede, N.S., Brownlie, A., Donovan, A., Liao, E.C., Drejer, A., Peters, L.L., Gwynn, B., Lux, S.E., and Zon, L.I. (2000) Cloning of the zebrafish Retsina blood mutation: Mutations in erythroid band 3 result in dyserythropoiesis and cytokinesis defects. Blood. 96(11):1895
Trede, N.S. and Zon, L.I. (1998) Development of T-cells during fish embryogenesis. Developmental and comparative immunology. 22:253-263

1. Trede, N.S., R. Urbanek, G. Burow, U. Wais: Vorfelddiagnostik zur Erfassung von Patienten mit Allergien der Atemwege. Helv. paediat. Acta 41, 243 (1986)
2. Otto,J. R. Urbanek, G. Burow, N. Trede, B. Müller: in-vitro Suchtest für die Diagnose von Allergien auf inhalative und nutritive Allergene. Mschr. Kinderheilkd. 134, 631 (1986)
3. Trede, N.S., R. Urbanek, G. Burow, J. Otto : Serological screening for allergy to inhalants by multi-allergen-radioimmunoassay. Helv. paediat. Acta 42, 129 (1987)
4. Trede, N.S., R. Urbanek : Combination of parenteral and oral immunotherapy in grass -pollenallergic children. A double-blind controlled study of clinical and immunological efficacy. Allergy 44, 272 (1989)
5. Jouvin-Marche, E., N.S. Trede, A. Bandeira, A. Tomas, D.Y. Loh, P.A. Cazenave : Different large deletions of T cell receptor V beta genes in natural populations of mice. Eur. J. Immunol. 19, 1921 (1989)
6. Jouvin-Marche, E., M.G. Morgado, N.S. Trede, P.N. Marche, D. Couez, I. Hue, C. Gris, M. Malissen, P.A. Cazenave : Complexity, polymorphism, and recombination of mouse T-cell receptor alpha gene families. Immunogenetics 30, 99 (1989)
7. Trede, N.S, R.S. Geha, T. Chatila : Transcriptional activation of IL-1<= and TNF-± genes by MHC class II ligands. J. Immunol. 146, 2310 (1991)
8. Scholl, P.R., N. Trede, T.A. Chatila, R.S. Geha: The role of tyrosine phosphorylation in monokine induction by the staphylococcal superantigen toxic shock syndrome toxin-1. J. Immunol. 148, 2237 (1992)
9. Trede, N.S., E. Castigli, R.S. Geha, T.A. Chatila: Microbial superantigens induce NF-?B in human monocytic cells. J. Immunol. 150, 5604 (1993)
10. Trede, N.S., P.R. Scholl, R.S. Geha, T.A. Chatila: Early activation events induced by the staphylococcal superantigen toxic-schock syndrome toxin-1 in human peripheral blood monocytes. Clin. Immunol. Immunopathol. 70, 137-144 (1994).
11. Fuleihan, R., N.S. Trede, T. Chatila, R.S. Geha: Superantigens activate HIV-1 gene expression in monocytes. Clin. Immunol. Immunopathol. 72, 357 (1994)
12. Trede, N.S., T.A. Chatila, R.S. Geha: Induction of activator protein-1 (AP-1) by staphylococcal superantigens in human monocytic cells. Europ. J. Immunol. 23, 2129 (1993)
13. Trede, N.S., A.V. Tsytsykova, A.E. Goldfeld, T. Chatila, R.S. Geha: Transcriptional activation of the human TNF-_ promoter by the microbial superantigen SEA in human monocytic cells. J. Immunol. 155, 902 (1995)
14. Trede, N.S., A.B.Warwick, P.M. Rosoff, B.E. Bierer, R. Rohrer, E.C. Guinan: Tacrolimus (FK506) in allogeneic bone marrow transplantation for severe aplastic anemia following orthotopic liver transplantation. Bone Marrow Transplantation, 20, 257 (1997)
15. Marler JJ, Rubin JB, Trede NS, Connors S, Grier H, Upton J, Mulliken JB, Folkman J.: Successful antiangiogenic therapy of giant cell angioblastoma with interferon alfa 2b: report of 2 cases. Pediatrics. 109, E37 (2002)

Urbanek, R., N.S. Trede : Orale Hyposensibilisierung. Fortschr. Med. 105, 415 (1987)
Chatila, T., P. Scholl, F. Spertini, N. Trede, R. Fuleihan, R.S. Geha : Toxic shock syndrome toxin-1, toxic shock and the immune system. Current Topics Microb. Immunol. 174, 63-80 (1991).
Chatila, T., P. Scholl, N. Ramesh, N. Trede, R. Fuleihan, T. Morio, R.S. Geha : Cellular and molecular mechanisms of immune activation by microbial superantigens: Studies using Toxic Shock Syndrome Toxin-1. Chem. Immunol. 55 , 146 (1992).