PUBLICATION

Resolution of the novel immune-type receptor gene cluster in zebrafish

Authors
Yoder, J.A., Litman, R.T., Mueller, M.G., Desai, S., Dobrinski, K.P., Montgomery, J.S., Buzzeo, M.P., Ota, T., Amemiya, C.T., Trede, N.S., Wei, S., Djeu, J.Y., Humphray, S., Jekosch, K., Hernandez Prada, J.A., Ostrov, D.A., and Litman, G.W.
ID
ZDB-PUB-041022-6
Date
2004
Source
Proceedings of the National Academy of Sciences of the United States of America   101(44): 15706-15711 (Journal)
Registered Authors
Amemiya, Chris, Dobrinski, Kim P., Howe (fka Jekosch), Kerstin, Humphray, Sean, Litman, Gary W., Montgomery, Jennifer, Ota, Tatsuya, Trede, Nick, Yoder, Jeffrey A.
Keywords
none
MeSH Terms
  • Alleles
  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Chromosome Mapping
  • DNA, Complementary/genetics
  • Evolution, Molecular
  • Genetic Variation
  • Lectins, C-Type/chemistry
  • Lectins, C-Type/genetics
  • Models, Molecular
  • Molecular Sequence Data
  • Multigene Family
  • Phylogeny
  • Protein Structure, Tertiary
  • Receptors, Immunologic/chemistry
  • Receptors, Immunologic/genetics*
  • Zebrafish/genetics*
  • Zebrafish/immunology*
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/immunology
PubMed
15496470 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
The novel immune-type receptor (NITR) genes encode a unique multigene family of leukocyte regulatory receptors, which possess an extracellular Ig variable (V) domain and may function in innate immunity. Artificial chromosomes that encode zebrafish NITRs have been assembled into a contig spanning approximately 350 kb. Resolution of the complete NITR gene cluster has led to the identification of eight previously undescribed families of NITRs and has revealed the presence of C-type lectins within the locus. A maximum haplotype of 36 NITR genes (138 gene sequences in total) can be grouped into 12 distinct families, including inhibitory and activating receptors. An extreme level of interindividual heterozygosity is reflected in allelic polymorphisms, haplotype variation, and family-specific isoform complexity. In addition, the exceptional diversity of NITR sequences among species suggests divergent evolution of this multigene family with a birth-and-death process of member genes. High-confidence modeling of Nitr V-domain structures reveals a significant shift in the spatial orientation of the Ig fold, in the region of highest interfamily variation, compared with Ig V domains. These studies resolve a complete immune gene cluster in zebrafish and indicate that the NITRs represent the most complex family of activating/inhibitory surface receptors thus far described.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes