PUBLICATION

NACA deficiency reveals the crucial role of somite-derived stromal cells in haematopoietic niche formation

Authors
Murayama, E., Sarris, M., Redd, M., Le Guyader, D., Vivier, C., Horsley, W., Trede, N., Herbomel, P.
ID
ZDB-PUB-150929-13
Date
2015
Source
Nature communications   6: 8375 (Journal)
Registered Authors
Herbomel, Philippe, Le Guyader, Dorothée, Murayama, Emi, Sarris, Milka, Trede, Nick, Vivier, Catherine
Keywords
Cell signalling, Epithelial-mesenchymal transition, Stem-cell niche
MeSH Terms
  • Animals
  • Apoptosis
  • Cell Survival
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/physiology*
  • Epithelial-Mesenchymal Transition*
  • Hematopoiesis
  • Hematopoietic Stem Cells/physiology*
  • Molecular Chaperones/physiology*
  • Mutation
  • Somites/cytology*
  • Zebrafish
PubMed
26411530 Full text @ Nat. Commun.
Abstract
The ontogeny of haematopoietic niches in vertebrates is essentially unknown. Here we show that the stromal cells of the caudal haematopoietic tissue (CHT), the first niche where definitive haematopoietic stem/progenitor cells (HSPCs) home in zebrafish development, derive from the caudal somites through an epithelial-mesenchymal transition (EMT). The resulting stromal cell progenitors accompany the formation of the caudal vein sinusoids, the other main component of the CHT niche, and mature into reticular cells lining and interconnecting sinusoids. We characterize a zebrafish mutant defective in definitive haematopoiesis due to a deficiency in the nascent polypeptide-associated complex alpha subunit (NACA). We demonstrate that the defect resides not in HSPCs but in the CHT niche. NACA-deficient stromal cell progenitors initially develop normally together with the sinusoids, and HSPCs home to the resulting niche, but stromal cell maturation is compromised, leading to a niche that is unable to support HSPC maintenance, expansion and differentiation.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes