NACA deficiency reveals the crucial role of somite-derived stromal cells in haematopoietic niche formation
- Murayama, E., Sarris, M., Redd, M., Le Guyader, D., Vivier, C., Horsley, W., Trede, N., Herbomel, P.
- Nature communications 6: 8375 (Journal)
- Registered Authors
- Herbomel, Philippe, Le Guyader, Dorothée, Murayama, Emi, Sarris, Milka, Trede, Nick, Vivier, Catherine
- Cell signalling, Epithelial-mesenchymal transition, Stem-cell niche
- MeSH Terms
- Cell Survival
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/physiology*
- Epithelial-Mesenchymal Transition*
- Hematopoietic Stem Cells/physiology*
- Molecular Chaperones/physiology*
- 26411530 Full text @ Nat. Commun.
Murayama, E., Sarris, M., Redd, M., Le Guyader, D., Vivier, C., Horsley, W., Trede, N., Herbomel, P. (2015) NACA deficiency reveals the crucial role of somite-derived stromal cells in haematopoietic niche formation. Nature communications. 6:8375.
The ontogeny of haematopoietic niches in vertebrates is essentially unknown. Here we show that the stromal cells of the caudal haematopoietic tissue (CHT), the first niche where definitive haematopoietic stem/progenitor cells (HSPCs) home in zebrafish development, derive from the caudal somites through an epithelial-mesenchymal transition (EMT). The resulting stromal cell progenitors accompany the formation of the caudal vein sinusoids, the other main component of the CHT niche, and mature into reticular cells lining and interconnecting sinusoids. We characterize a zebrafish mutant defective in definitive haematopoiesis due to a deficiency in the nascent polypeptide-associated complex alpha subunit (NACA). We demonstrate that the defect resides not in HSPCs but in the CHT niche. NACA-deficient stromal cell progenitors initially develop normally together with the sinusoids, and HSPCs home to the resulting niche, but stromal cell maturation is compromised, leading to a niche that is unable to support HSPC maintenance, expansion and differentiation.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes