Zebrafish mutants with disrupted early T-cell and thymus development identified in early pressure screen
- Trede, N.S., Ota, T., Kawasaki, H., Paw, B.H., Katz, T., Demarest, B., Hutchinson, S., Zhou, Y., Hersey, C., Zapata, A., Amemiya, C.T., and Zon, L.I.
- Developmental dynamics : an official publication of the American Association of Anatomists 237(9): 2575-2584 (Journal)
- Registered Authors
- Amemiya, Chris, Demarest, Bradley, Hersey, Candace, Ota, Tatsuya, Paw, Barry, Trede, Nick, Zhou, Yi, Zon, Leonard I.
- zebrafish, mutagenesis screen, lymphocyte, thymus, pharyngeal arch
- MeSH Terms
- Branchial Region/embryology
- Branchial Region/metabolism
- Forkhead Transcription Factors/genetics
- Gene Expression Regulation, Developmental*
- Homeodomain Proteins/genetics
- Ikaros Transcription Factor/genetics
- In Situ Hybridization
- Thymus Gland/cytology
- Thymus Gland/embryology
- Thymus Gland/metabolism*
- Zebrafish Proteins/genetics
- 18729230 Full text @ Dev. Dyn.
Trede, N.S., Ota, T., Kawasaki, H., Paw, B.H., Katz, T., Demarest, B., Hutchinson, S., Zhou, Y., Hersey, C., Zapata, A., Amemiya, C.T., and Zon, L.I. (2008) Zebrafish mutants with disrupted early T-cell and thymus development identified in early pressure screen. Developmental dynamics : an official publication of the American Association of Anatomists. 237(9):2575-2584.
Generation of mature T lymphocytes requires an intact hematopoietic stem cell compartment and functional thymic epithelium. We used the zebrafish (Danio rerio) to isolate mutations that affect the earliest steps in T lymphopoiesis and thymic organogenesis. Here we describe the results of a genetic screen in which gynogenetic diploid offspring from heterozygous females were analyzed by whole-mount in situ hybridization for the expression of rag-1. To assess immediately if a global defect in hematopoiesis resulted in the mutant phenotype, alpha-embryonic globin expression was simultaneously assayed for multilineage defects. In this report, we present the results obtained with this strategy and show representative mutant phenotypes affecting early steps in T-cell development and/or thymic epithelial cell development. We discuss the advantage of this strategy and the general usefulness of the zebrafish as a model system for vertebrate lymphopoiesis and thymic organogenesis.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes