ZFIN ID: ZDB-PUB-030211-10
Myc-induced T cell leukemia in transgenic zebrafish
Langenau, D.M., Traver, D., Ferrando, A.A., Kutok, J.L., Aster, J.C., Kanki, J.P., Lin, S., Prochownik, E., Trede, N.S., Zon, L.I., and Look, A.T.
Date: 2003
Source: Science (New York, N.Y.) 299(5608): 887-890 (Journal)
Registered Authors: Kanki, John, Langenau, David, Lin, Shuo, Look, A. Thomas, Traver, David, Trede, Nick, Zon, Leonard I.
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Lineage
  • Cell Transformation, Neoplastic*
  • Clone Cells
  • DNA-Binding Proteins/genetics
  • Disease Models, Animal*
  • Female
  • Fertilization in Vitro
  • Gene Expression Profiling
  • Genes, myc*
  • Green Fluorescent Proteins
  • Kidney/pathology
  • Leukemia-Lymphoma, Adult T-Cell*/genetics
  • Leukemia-Lymphoma, Adult T-Cell*/pathology
  • Leukemic Infiltration
  • Luminescent Proteins/metabolism
  • Male
  • Mice
  • Mutation
  • Neoplasm Transplantation
  • Olfactory Bulb/pathology
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins/metabolism
  • Spleen/pathology
  • T-Lymphocytes/immunology
  • T-Lymphocytes/pathology*
  • T-Lymphocytes/physiology
  • Thymus Gland/pathology
  • Transgenes
  • Zebrafish*/embryology
  • Zebrafish*/genetics
PubMed: 12574629 Full text @ Science
ABSTRACT
The zebrafish is an attractive model organism for studying cancer development because of its genetic accessibility. Here we describe the induction of clonally derived T cell acute lymphoblastic leukemia in transgenic zebrafish expressing mouse c-myc under control of the zebrafish Rag2 promoter. Visualization of leukemic cells expressing a chimeric transgene encoding Myc fused to green fluorescent protein (GFP) revealed that leukemias arose in the thymus, spread locally into gill arches and retro-orbital soft tissue, and then disseminated into skeletal muscle and abdominal organs. Leukemic cells homed back to the thymus in irradiated fish transplanted with GFP-labeled leukemic lymphoblasts. This transgenic model provides a platform for drug screens and for genetic screens aimed at identifying mutations that suppress or enhance c-myc- induced carcinogenesis.
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