Myc-induced T cell leukemia in transgenic zebrafish
- Langenau, D.M., Traver, D., Ferrando, A.A., Kutok, J.L., Aster, J.C., Kanki, J.P., Lin, S., Prochownik, E., Trede, N.S., Zon, L.I., and Look, A.T.
- Science (New York, N.Y.) 299(5608): 887-890 (Journal)
- Registered Authors
- Kanki, John, Langenau, David, Lin, Shuo, Look, A. Thomas, Traver, David, Trede, Nick, Zon, Leonard I.
- MeSH Terms
- Animals, Genetically Modified
- Cell Lineage
- Cell Transformation, Neoplastic*
- Clone Cells
- DNA-Binding Proteins/genetics
- Disease Models, Animal*
- Fertilization in Vitro
- Gene Expression Profiling
- Genes, myc*
- Green Fluorescent Proteins
- Leukemia-Lymphoma, Adult T-Cell*/genetics
- Leukemia-Lymphoma, Adult T-Cell*/pathology
- Leukemic Infiltration
- Luminescent Proteins/metabolism
- Neoplasm Transplantation
- Olfactory Bulb/pathology
- Promoter Regions, Genetic
- Recombinant Fusion Proteins/metabolism
- Thymus Gland/pathology
- 12574629 Full text @ Science
Langenau, D.M., Traver, D., Ferrando, A.A., Kutok, J.L., Aster, J.C., Kanki, J.P., Lin, S., Prochownik, E., Trede, N.S., Zon, L.I., and Look, A.T. (2003) Myc-induced T cell leukemia in transgenic zebrafish. Science (New York, N.Y.). 299(5608):887-890.
The zebrafish is an attractive model organism for studying cancer development because of its genetic accessibility. Here we describe the induction of clonally derived T cell acute lymphoblastic leukemia in transgenic zebrafish expressing mouse c-myc under control of the zebrafish Rag2 promoter. Visualization of leukemic cells expressing a chimeric transgene encoding Myc fused to green fluorescent protein (GFP) revealed that leukemias arose in the thymus, spread locally into gill arches and retro-orbital soft tissue, and then disseminated into skeletal muscle and abdominal organs. Leukemic cells homed back to the thymus in irradiated fish transplanted with GFP-labeled leukemic lymphoblasts. This transgenic model provides a platform for drug screens and for genetic screens aimed at identifying mutations that suppress or enhance c-myc- induced carcinogenesis.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes