ZFIN ID: ZDB-PUB-120412-5
Zebrafish screen identifies novel compound with selective toxicity against leukemia
Ridges, S., Heaton, W.L., Joshi, D., Choi, H., Eiring, A., Batchelor, L., Choudhry, P., Manos, E.J, Sofla, H., Sanati, A., Welborn, S., Agarwal, A., Spangrude, G.J., Miles, R.R., Cox, J.E., Frazer, J.K., Deininger, M., Balan, K., Sigman, M., Müschen, M., Perova, T., Johnson, R., Montpellier, B., Guidos, C.J., Jones, D.A., and Trede, N.S.
To detect targeted anti-leukemia agents we have designed a novel, high-content in vivo screen using genetically engineered, T cell-reporting zebrafish. We exploited the developmental similarities between normal
and malignant T lymphoblasts to screen a small molecule library for activity against immature T cells with a simple visual
read-out in zebrafish larvae. After screening 26,400 molecules, we identified Lenaldekar (LDK), a compound that eliminates
immature T cells in developing zebrafish without affecting the cell cycle in other cell types. LDK is well tolerated in vertebrates
and induces long-term remission in adult zebrafish with cMYC-induced T cell acute lymphoblastic leukemia (T-ALL). LDK causes
dephosphorylation of members of the PI3 kinase/AKT/mTOR pathway and delays sensitive cells in late mitosis. Among human cancers,
LDK selectively affects survival of hematopoietic malignancy lines and primary leukemias, including therapy-refractory B-ALL
and CML samples, and inhibits growth of human T-ALL xenografts. This work demonstrates the utility of our method using zebrafish
for anti-neoplastic candidate drug identification and suggests a new approach for targeted leukemia therapy. While our efforts
focused on leukemia therapy, this screening approach has broad implications as it can be translated to other cancer types
involving malignant degeneration of developmentally arrested cells.