In vivo tracking of T cell development, ablation, and engraftment in transgenic zebrafish
- Langenau, D.M., Ferrando, A.A., Traver, D., Kutok, J.L., Hezel, J.P., Kanki, J.P., Zon, L.I., Look, A.T., and Trede, N.S.
- Proceedings of the National Academy of Sciences of the United States of America 101(19): 7369-7374 (Journal)
- Registered Authors
- Kanki, John, Langenau, David, Look, A. Thomas, Traver, David, Trede, Nick, Zon, Leonard I.
- MeSH Terms
- Animals, Genetically Modified/immunology*
- In Situ Hybridization
- Molecular Sequence Data
- Reverse Transcriptase Polymerase Chain Reaction
- T-Lymphocytes/drug effects
- 15123839 Full text @ Proc. Natl. Acad. Sci. USA
Langenau, D.M., Ferrando, A.A., Traver, D., Kutok, J.L., Hezel, J.P., Kanki, J.P., Zon, L.I., Look, A.T., and Trede, N.S. (2004) In vivo tracking of T cell development, ablation, and engraftment in transgenic zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 101(19):7369-7374.
Transgenic zebrafish that express GFP under control of the T cell-specific tyrosine kinase (lck) promoter were used to analyze critical aspects of the immune system, including patterns of T cell development and T cell homing after transplant. GFP-labeled T cells could be ablated in larvae by either irradiation or dexamethasone added to the water, illustrating that T cells have evolutionarily conserved responses to chemical and radiation ablation. In transplant experiments, thymocytes from lck-GFP fish repopulated the thymus of irradiated wild-type fish only transiently, suggesting that the thymus contains only short-term thymic repopulating cells. By contrast, whole kidney marrow permanently reconstituted the T lymphoid compartment of irradiated wild-type fish, suggesting that long-term thymic repopulating cells reside in the kidney.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes