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Fig. 5 The development of the liver was abnormal in the <italic>mpv17</italic><sup><italic>−/−</italic></sup> zebrafish.

A In situ hybridization with RNA probe tfa at 3 dpf. Wild-type was in the upper, mpv17−/− in the middle, and the lower was the mpv17−/− larvae rescued with mpv17 mRNA. B In situ hybridization of larvae at 5 dpf using tfa probe. C The expression level of genes tfa. Error bars were indicated with ±SEM, *p< 0.05. D The expression levels of genes involved in liver development. Error bars were indicated with ±SEM, **p< 0.01, ***p< 0.005. E H&E staining paraffin-embedded sections of the liver in 1-month-old zebrafish. The black frames were enlarged as shown on the right.

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Acknowledgments
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