FIGURE

Figure 8

ID

ZDB-FIG-221008-36

Publication

Xing et al., 2022 - Expression of myelin transcription factor 1 and lamin B receptor mediate neural progenitor fate transition in the zebrafish spinal cord pMN domain

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Figure 8

Cell culture of neural stem cells dissected from mouse brain.A–C, morphology of cells transfected with a scrambled control (shNC, A) and Lbr knock down lentivirus (shLbr-1 and shLbr-2, B and C). The scale bar represents 100 μm. D, the enlarged images from the boxed area in (C). The scale bar represents 25 μm. E and F, quantification of cells with processes (E) and the number of neurosphere formation (F). G, CCK8 analysis of cells transfected with Lbr lentivirus. H, Western blot reveals a dramatic decrease of LBR expression in cells transfected with shLbr-1 or shLbr-2. I–L, qPCR analysis of stemness-related genes. Data were shown as the mean ± SD. ∗∗, ∗∗∗, and ∗∗∗∗ denote p < 0.01, p < 0.001, p < 0.0001, respectively.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biol. Chem.