PUBLICATION
Expression of myelin transcription factor 1 and lamin B receptor mediate neural progenitor fate transition in the zebrafish spinal cord pMN domain
- Authors
- Xing, L., Chai, R., Wang, J., Lin, J., Li, H., Wang, Y., Lai, B., Sun, J., Chen, G.
- ID
- ZDB-PUB-220907-22
- Date
- 2022
- Source
- The Journal of biological chemistry 298(10): 102452 (Journal)
- Registered Authors
- Keywords
- interneurons, motor neurons, oligodendrocyte progenitor cells (OPCs), pMN progenitor cells, radial glia, single-cell RNA sequencing (scRNA-seq), zebrafish
- Datasets
- GEO:GSE179096, GEO:GSE186163
- MeSH Terms
-
- Animals
- Basic Helix-Loop-Helix Transcription Factors*/genetics
- Basic Helix-Loop-Helix Transcription Factors*/metabolism
- Cell Differentiation/physiology
- Myelin Sheath/metabolism
- Nerve Tissue Proteins/metabolism
- Oligodendrocyte Transcription Factor 2/metabolism
- Oligodendroglia/metabolism
- Receptors, Cytoplasmic and Nuclear/genetics
- Receptors, Cytoplasmic and Nuclear/metabolism
- Spinal Cord/metabolism
- Transcription Factors*/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 36063998 Full text @ J. Biol. Chem.
Citation
Xing, L., Chai, R., Wang, J., Lin, J., Li, H., Wang, Y., Lai, B., Sun, J., Chen, G. (2022) Expression of myelin transcription factor 1 and lamin B receptor mediate neural progenitor fate transition in the zebrafish spinal cord pMN domain. The Journal of biological chemistry. 298(10):102452.
Abstract
The pMN domain is a restricted domain in the ventral spinal cord, defined by the expression of the olig2 gene. Though it is known that the pMN progenitor cells can sequentially generate motor neurons and oligodendrocytes, the lineages of these progenitors are controversial and how their progeny are generated is not well understood. Using single-cell sequencing (scRNA-seq), here we identified a previously unknown heterogeneity among pMN progenitors with distinct fates and molecular signatures in zebrafish. Notably, we characterized two distinct motor neuron lineages using bioinformatic analysis. We then went on to investigate specific molecular programs that regulate neural progenitor fate transition. We validated experimentally that expression of the transcription factor myt1 (myelin transcription factor 1) and inner nuclear membrane integral proteins lbr (lamin B receptor) were critical for the development of motor neurons and neural progenitor maintenance, respectively. We anticipate that the transcriptome features and molecular programs identified in zebrafish pMN progenitors will not only provide an in-depth understanding of previous findings regarding the lineage analysis of oligodendrocyte progenitor cells and motor neurons, but will also help to further our understanding of the molecular programming involved in neural progenitor fate transition.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping