FIGURE

Fig. 11

ID

ZDB-FIG-220622-35

Publication

Fleming et al., 2022 - Unexpected Phenotype Reversion and Survival in a Zebrafish Model of Multiple Sulfatase Deficiency

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Fig. 11

FIGURE 11. Sulfatase activity in older larvae and adult brains. Larvae at 5 d.p.f. showed no or neglible sulfatase activity (Figure 2B). However, since pathological phenotypes in sumf1^−/− larvae resolved with age and adult sumf1^−/−fish were viable, we measured sulfatase activity at older timepoints. No or negligible levels of ARSA and GALNS activity could be detected in sumf1^−/− larvae at 10 and 15 d.p.f., and in the brains of adult fish. Beta-galactosidase was included as a control as this enzyme is a lysosomal hydrolase which is not dependent on FGE. Graphs show mean values (±SD) from at least 3 biological replicates. Statistical analysis was performed using a two-tailed t-test. p > 0.05, *: p ≤ 0.05, **: p ≤ 0.01, and ***: p ≤ 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sumf1^{la015919Tg/la015919Tg} (TL)
Observed In:	brain cerebroside-sulfatase activity brain N-acetylgalactosamine-6-sulfatase activity whole organism beta-galactosidase activity whole organism cerebroside-sulfatase activity whole organism N-acetylgalactosamine-6-sulfatase activity
Stage Range:	Days 7-13 to Adult

Phenotype Detail

Acknowledgments

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