ZFIN Image: Fleming et al., 2022, Fig. 11

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Figure Caption

Fig. 11

FIGURE 11. Sulfatase activity in older larvae and adult brains. Larvae at 5 d.p.f. showed no or neglible sulfatase activity (Figure 2B). However, since pathological phenotypes in sumf1^−/− larvae resolved with age and adult sumf1^−/−fish were viable, we measured sulfatase activity at older timepoints. No or negligible levels of ARSA and GALNS activity could be detected in sumf1^−/− larvae at 10 and 15 d.p.f., and in the brains of adult fish. Beta-galactosidase was included as a control as this enzyme is a lysosomal hydrolase which is not dependent on FGE. Graphs show mean values (±SD) from at least 3 biological replicates. Statistical analysis was performed using a two-tailed t-test. p > 0.05, *: p ≤ 0.05, **: p ≤ 0.01, and ***: p ≤ 0.001.

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Phenotype details

Acknowledgments

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