FIGURE

FIGURE 1

ID

ZDB-FIG-220402-18

Publication

Brożko et al., 2022 - Genoarchitecture of the Early Postmitotic Pretectum and the Role of Wnt Signaling in Shaping Pretectal Neurochemical Anatomy in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 1

Identification of pretectal boundaries in the diencephalon of 48 hpf zebrafish. Confocal Z-stack images showing brain sections stained using in situ hybridization with nkx2.2a, lhx9, tcf7l2 and gbx2 probes. Sagittal sections: (A–D) Expression of nkx2.2a and lhx9 in medial (A,B) and lateral (C,D) sections delineate the alar-basal boundary. Also, nkx2.2a expression identifies the rTh and Pth, while lhx9 expression identifies the cTh and Hab. (E,F) Expression of tcf7l2 identifies prosomere 1 and 2; co-staining of tcf7l2 and gbx2 identifies the cTh. Transverse sections:(G,H)tcf7l2 co-stained with gbx2 and nkx2.2a marks the Pt, Hab, cTh, and rTh. The schemes show the regions identified by the markers in each merged image. Stripe pattern indicates co-expression of two markers; saturated color indicates high expression signal while lighter color indicates low signal. The yellow lines in (E,F) show the sectioning plane of the transverse sections in (G,H). Ap, alar plate; bp, basal plate; Hab, habenula; Mes, mesencephalon; p1, prosomere 1; p2, prosomere 2; p3, prosomere 3; Pt, pretectum; Pth, prethalamus; Rh, rhombencephalon; cTh, caudal thalamus; rTh, rostral thalamus.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neuroanat.