ZFIN ID: ZDB-FIG-200713-37
Peron et al., 2020 - The stem-like STAT3-responsive cells of zebrafish intestine are WNT/β-catenin dependent. Development (Cambridge, England)   147(12) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Day 6 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 6 to Adult

Fig. 7.

Tcf7l2 (Tcf4) is required for development of Stat3-responsive cells of zebrafish larvae intestine and the Stat3 pathway is activated ectopically in intestinal adenomas of apchu745 mutants. (A,A′) In vivo EGFP fluorescence in the intestine of 6 dpf Tg(7xStat3:EGFP)/tcf7l2hu892/hu892, Tg(7xStat3:EGFP)/tcf7l2+/hu892 and Tg(7xStat3:EGFP)/tcf7l2+/+ siblings (A) and measurement of integrated density (A′) (n=16). (B) qPCR analysis of il6, gp130, jak2a, jak2b and stat3 mRNA expression from tcf7l2+/+ and tcf7l2hu892/hu892 sibling larvae. (C,C′) Effect of XAV treatment on Tg(7xStat3:EGFP) embryos from 48 to 78 hpf: measurement of the integrated density of the fluorescence (C) and measurement of the number of GFP+ cells (C′). (D,E) Haematoxylin-eosin staining on paraffin embedded transversal section of zebrafish apchu745 intestine at 12 months post fertilization, displaying both normal tissue (n) and hyperplastic adenomas (a). (D′,D″) Staining of a sequential intestinal section of D using α-EGFP (green) (D′) and α-PCNA (red) (D″) Abs. (E′,E″) Double staining using α-EGFP Ab (green) and α-PCNA Ab (red) of a sequential intestinal section of E. All statistical analyses were performed by unpaired t-test. *P<0.05, ***P<0.001; ns, not significant. Error bars indicate s.e.m.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP apchu745/+; ia28Tg standard conditions Adult intestine hyperplastic polyp IHC
apchu745/hu745; ia28Tg standard conditions Day 6 intestine IFL
ia28Tg standard conditions Day 6 intestine IFL
il6 WT standard conditions Days 7-13 whole organism RTPCR
tcf7l2zf55/zf55 standard conditions Days 7-13 whole organism RTPCR
il6st WT standard conditions Days 7-13 whole organism RTPCR
tcf7l2zf55/zf55 standard conditions Days 7-13 whole organism RTPCR
jak2a WT standard conditions Days 7-13 whole organism RTPCR
tcf7l2zf55/zf55 standard conditions Days 7-13 whole organism RTPCR
jak2b WT standard conditions Days 7-13 whole organism RTPCR
tcf7l2zf55/zf55 standard conditions Days 7-13 whole organism RTPCR
stat3 WT standard conditions Days 7-13 whole organism RTPCR
tcf7l2zf55/zf55 standard conditions Days 7-13 whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-pcna IHC apchu745/+; ia28Tg standard conditions Adult intestine hyperplastic polyp
Phenotype Details
Fish Conditions Stage Phenotype
apchu745/+; ia28Tg standard conditions Adult intestine adenomatous polyp increased amount, abnormal
Adult intestine hyperplastic polyp ab2-pcna labeling increased distribution, abnormal
Adult intestine hyperplastic polyp EGFP expression increased distribution, abnormal
apchu745/hu745; ia28Tg standard conditions Day 6 intestine EGFP expression decreased distribution, abnormal
tcf7l2zf55/zf55 standard conditions Days 7-13 whole organism jak2a expression decreased amount, abnormal
Days 7-13 whole organism stat3 expression decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development