FIGURE

Fig. 6-S1

ID
ZDB-FIG-191014-24
Publication
Yu et al., 2019 - Stochastic cell-cycle entry and cell-state-dependent fate outputs of injury-reactivated tectal radial glia in zebrafish
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Fig. 6-S1

The injury wounds are failed to be restored.

(A–B3) Representative images of EdU (red), HuC/D (green) and BLBP (gray) immunofluorescences showing a EdU+/HuC/D+ neuron migrates to the upper region of injured optic tectum at 300 dpi. (B–B3) The high-magnification images of boxed area in (A). White arrowheads indicate the EdU+/HuC/D+ neuron. Yellow dashed lines indicate the RG layer. White dashed lines and white asterisks indicate the stab wound that is not restored at 300 dpi. (C–D3) Representative images of EdU (red), HuC/D (green) and BLBP (gray) immunofluorescences showing the processes terminal of RG near the injury site are hypertrophic with high level of BLBP expression at 7 dpi. (D–D3) The representative images of uninjured optic tectum. (E) Quantification of the area of stab wounds at 1–4 (1343 ± 315.7 μm2) and 300–400 dpi (1339 ± 768.6 μm2) showing that the stab wounds are not completely restored (mean ± SEM, ns, p>0.05; Wilcoxon test). (F–I3) Representative images of EdU (red), HuC/D (green) and BLBP (gray) immunofluorescences showing stab wound that is surrounded by hypertrophic RG processes terminal (high level of BLBP expression, gray signals) is remained at 300 dpi. (H–H3) are the representative images of uninjured optic tectum. (G–G3) and (I–I3) The high-magnification images of boxed areas in (F) and (H), respectively. (J and K) t-SNE and violin plots showing that hmgb2a and hmgb2b are enriched in proliferative-S and -G2 RG. (L–M2) In situ hybridization showing PCNA+ RG (green cells) express hmgb2a (red signals) at 3 dpi. (M–M2) are the representative images of uninjured optic tectum. Yellow arrow heads indicate the injury site. RG, radial glia; TeO, tectal opticum; PGZ, periventricular gray zone; TS, torus semicircularis; Val, valvula cerebelli. Scale bars, 50 μm (A, F and H); 30 μm (B–B3, C–C3, D–D3, G–G3, I–I3 and L–M2).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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