ZFIN ID: ZDB-FIG-190923-9
Chambers et al., 2019 - Tfap2a is a novel gatekeeper of nephron differentiation during kidney development. Development (Cambridge, England)   146(13) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 2

tfap2a and tfap2b are expressed in dynamic overlapping domains in developing nephrons, where tfap2a acts upstream of tfap2b. (A) Whole-mount in situ hybridization for tfap2a and tfap2b expression (purple), and smyhc1 expression (red). Black boxes indicate pronephros expression. Scale bar: 200 µm. (B) Fluorescent in situ hybridization for tfap2a (green) and tfap2b(red) in 10 ss (flat mount), 20 ss and 28 ss wild-type embryos (lateral views). White arrowheads indicate overlap. Scale bar: 70 µm. (C) Fluorescent in situ hybridization for pax2a (green), tfap2a (red) and tfap2b (red) in 10 ss wild-type embryos. White boxes outline the region shown in more detail in the bottom panel. Cyan circles indicate example cells co-expressing pax2a and tfap2a or tfap2b. White dots indicate the renal progenitor field border. Scale bars: 100 µm (top) and 10 µm (bottom). (D) Fluorescent in situ hybridization for slc12a1 (magenta) and tfap2a (green). White box indicates the area shown in more detail below. Scale bars: 70 µm (top) and 10 µm (bottom). (E) Whole-mount in situ hybridization for tfap2b. Arrowhead indicates hindbrain. Scale bars: 100 µm (left) and 70 µm (right). (F) Absolute length quantification of tfap2b. (G) Whole-mount in situ hybridization for tfap2a. Scale bars: 70 µm. (H) Absolute length quantification of tfap2an≥3. Measurements compared using unpaired t-tests. Data are mean±s.d. ***P<0.001; N.S., not significant.

 

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
pax2a TU standard conditions 10-13 somites pronephros distal region ISH
slc12a1 TU standard conditions 26+ somites pronephric distal early tubule ISH
smyhc1 TU standard conditions 10-13 somites somite ISH
14-19 somites somite ISH
20-25 somites somite ISH
26+ somites somite ISH
tfap2a TU standard conditions 10-13 somites pronephros distal region ISH
14-19 somites pronephros distal region ISH
20-25 somites posterior pronephric duct ISH
20-25 somites pronephros distal region ISH
26+ somites posterior pronephric duct ISH
26+ somites pronephric distal early tubule ISH
26+ somites pronephros distal region ISH
Prim-5 pronephros distal region ISH
tfap2bsa10090/sa10090 standard conditions Prim-5 pronephros distal region ISH
tfap2b TU standard conditions 10-13 somites pronephros distal region ISH
14-19 somites pronephros distal region ISH
20-25 somites Not Detected posterior pronephric duct ISH
20-25 somites pronephros distal region ISH
26+ somites Not Detected posterior pronephric duct ISH
26+ somites pronephros distal region ISH
Prim-5 hindbrain ISH
Prim-5 pronephros distal region ISH
tfap2ant30/nt30 standard conditions Prim-5 hindbrain ISH
Prim-5 pronephros distal region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tfap2ant30/nt30 standard conditions Prim-5 hindbrain tfap2b expression decreased amount, abnormal
Prim-5 pronephros distal region tfap2b expression decreased amount, abnormal
Prim-5 pronephros distal region tfap2b expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development