ZFIN ID: ZDB-FIG-190923-11
Chambers et al., 2019 - Tfap2a is a novel gatekeeper of nephron differentiation during kidney development. Development (Cambridge, England)   146(13) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-5

Fig. 4

tfap2a is necessary and sufficient to drive the DE gene expression program. (A) Whole-mount in situ hybridization with the black bar indicating the kcnj1a.1 domain. Scale bars: 70 µm. (B) Absolute quantification of domain length per nephron. n≥10. Measurements compared by ANOVA. Data are mean±s.d. ***P<0.001; N.S., not significant. HS+, heat-shock; HS, no heat-shock. +tfap2a cRNA (blue) indicates RNA microinjection. (C) Fluorescent in situ hybridization for indicated markers. Scale bar: 20 µm. Cyan arrowheads identify ectopic slc12a1 in an adjacent segment. Cyan box indicates area shown in C′. (C′) DAPI (left) and merge (right). White dots outline a cell co-expressing slc12a1 and slc12a3. Scale bar: 5 µm. (D) Fluorescent intensity plot of slc12a1 expression in individuals from C. Blue dashed line demarcates wild-type segment boundary location. Purple arrows indicate ectopic slc12a1 in an adjacent segment and correlate with ectopic slc12a1 identified by cyan arrows in C. (E) Fluorescent in situ hybridization for indicated markers. Scale bar: 35 µm. Cyan arrowheads identify ectopic slc12a1 in an adjacent segment. Cyan box indicates the area shown in E′. (E′) DAPI (left) and merge (right). White dots outline cell co-expressing slc9a3 and slc12a1. Scale bar: 5 µm. DAPI (blue) labels nuclei. (F) Fluorescent intensity plot of slc12a1 expression in individuals from E. Blue dashed line represents the wild-type segment boundary. Purple arrows indicate ectopic slc12a1 in an adjacent segment and correlate with ectopic slc12a1 (cyan arrowheads) in E. White dotted lines throughout demarcate the pronephros; all embryos are at 24 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
kcnj1a.1 TU standard conditions Prim-5 pronephric distal early tubule ISH
tfap2ant30/nt30 standard conditions Prim-5 pronephric distal early tubule ISH
x24Tg heat shock Prim-5 pronephric distal early tubule ISH
x24Tg ; tfap2ant30/nt30 standard conditions Prim-5 pronephric distal early tubule ISH
x24Tg ; tfap2ant30/nt30 heat shock Prim-5 pronephric distal early tubule ISH
slc9a3r1a TU standard conditions Prim-5 pronephric proximal straight tubule ISH
x24Tg heat shock Prim-5 pronephric proximal straight tubule ISH
slc12a1 TU standard conditions Prim-5 pronephric distal early tubule ISH
x24Tg heat shock Prim-5 pronephric distal early tubule ISH
Prim-5 pronephric distal late tubule ISH
Prim-5 pronephric proximal straight tubule ISH
slc12a3 TU standard conditions Prim-5 pronephric distal late tubule ISH
x24Tg heat shock Prim-5 pronephric distal early tubule ISH
Prim-5 pronephric distal late tubule ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tfap2ant30/nt30 standard conditions Prim-5 pronephric distal early tubule kcnj1a.1 expression decreased amount, abnormal
Prim-5 pronephric distal early tubule kcnj1a.1 expression decreased distribution, abnormal
Prim-5 pronephric distal early tubule pronephric nephron tubule epithelial cell differentiation decreased occurrence, abnormal
x24Tg heat shock Prim-5 pronephric distal early tubule kcnj1a.1 expression increased distribution, abnormal
Prim-5 pronephric distal early tubule slc12a3 expression mislocalised, abnormal
Prim-5 pronephric distal early tubule pronephric nephron tubule epithelial cell differentiation occurrence, abnormal
Prim-5 pronephric distal late tubule slc12a1 expression mislocalised, abnormal
Prim-5 pronephric proximal straight tubule slc12a1 expression mislocalised, abnormal
x24Tg ; tfap2ant30/nt30 standard conditions Prim-5 pronephric distal early tubule kcnj1a.1 expression decreased amount, abnormal
Prim-5 pronephric distal early tubule kcnj1a.1 expression decreased distribution, abnormal
Prim-5 pronephric distal early tubule pronephric nephron tubule epithelial cell differentiation decreased occurrence, abnormal
x24Tg ; tfap2ant30/nt30 heat shock Prim-5 pronephric distal early tubule kcnj1a.1 expression amount, ameliorated
Prim-5 pronephric distal early tubule kcnj1a.1 expression position, ameliorated
Prim-5 pronephric distal early tubule pronephric nephron tubule epithelial cell differentiation occurrence, ameliorated
Prim-5 pronephric distal late tubule pronephric nephron tubule epithelial cell differentiation occurrence, abnormal
Prim-5 pronephric proximal straight tubule pronephric nephron tubule epithelial cell differentiation occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development