ZFIN ID: ZDB-FIG-190918-2
Isles et al., 2019 - The CXCL12/CXCR4 Signaling Axis Retains Neutrophils at Inflammatory Sites in Zebrafish. Frontiers in immunology   10:1784 Full text @ Front Immunol
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Protruding-mouth to Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 1

cxcr4b and cxcl12a are expressed following tissue damage in zebrafish. (A–D) Single-cell gene expression profiles for cxcr4 and cxcl12 in the zebrafish blood lineage. Single cell gene expression values extracted from the Sanger BASiCz zebrafish blood atlas. Circles represent individual cells color coded where red is high expression and yellow is no expression. Neutrophil lineage (mpx:GFP positive) is highlighted by black dashed box and expanded in (i–iv). (E,F) RNA sequencing of FACS sorted GFP positive cells from TgBAC(mpx:GFP)i114 zebrafish larvae at 5 days post fertilization. FPKM values illustrate neutrophil expression of (E) cxcr4a and cxcr4b and (F)cxcl12a and cxcl12b(G) Whole mount in situ hybridization using an antisense DIG labeled RNA probe for cxcl12a mRNA. Wildtype nacre zebrafish larvae were injured and fixed in PFA at 6, 12, and 24 h post injury, along with uninjured age-matched control larvae. Left and middle panels show whole zebrafish larvae at timepoints indicated, right panel shows tail fins of a representative experiment. Quantification shows number of larvae which look like representative image from 2 independent experiments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cxcl12a i114Tg ; mitfaw2/w2 standard conditions Adult neutrophil OTHR
mitfaw2/w2 standard conditions Protruding-mouth caudal hematopoietic tissue ISH
Protruding-mouth head ISH
Protruding-mouth pronephric duct ISH
Day 4 caudal hematopoietic tissue ISH
Day 4 head ISH
Day 4 pronephric duct ISH
mitfaw2/w2 amputation: caudal fin Protruding-mouth caudal fin ISH
Protruding-mouth caudal hematopoietic tissue ISH
Protruding-mouth head ISH
Protruding-mouth pronephric duct ISH
Day 4 caudal fin ISH
Day 4 caudal hematopoietic tissue ISH
Day 4 head ISH
Day 4 pronephric duct ISH
cxcl12b i114Tg ; mitfaw2/w2 standard conditions Adult blood OTHR
cxcr4a i114Tg ; mitfaw2/w2 standard conditions Adult Not Detected neutrophil OTHR
cxcr4b i114Tg ; mitfaw2/w2 standard conditions Adult neutrophil OTHR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mitfaw2/w2 amputation: caudal fin Protruding-mouth caudal fin cxcl12a expression increased amount, abnormal
Day 4 caudal fin cxcl12a expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in immunology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front Immunol