Expression of genes involved in lymphatic specification. (A–H) Relative expression levels (at 24 and 36 hpf) of genes involved in lymphatic specification were quantified by RT-qPCR in morphants injected with Cont MO or EP3 MO1. Values are shown relative to the value obtained with Cont MO at 24 hpf. Each value represents the mean ± SEM (N = 3). *P < 0.05, **P < 0.01 vs Cont MO at each corresponding time. (I,J) Expression of nr2f2 was analyzed by WISH in morphants at 24 and 36 hpf. The number at the bottom right of each panel indicates the number of embryos demonstrating the phenotype shown in the panel over the total number of embryos analyzed in a representative experiment.