ZFIN ID: ZDB-FIG-190723-255
Iwasaki et al., 2019 - Essential role of prostaglandin E2 and the EP3 receptor in lymphatic vessel development during zebrafish embryogenesis. Scientific Reports   9:7650 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Prim-25

Figure 3

Expression of genes involved in lymphatic specification. (AH) Relative expression levels (at 24 and 36 hpf) of genes involved in lymphatic specification were quantified by RT-qPCR in morphants injected with Cont MO or EP3 MO1. Values are shown relative to the value obtained with Cont MO at 24 hpf. Each value represents the mean ± SEM (N = 3). *P < 0.05, **P < 0.01 vs Cont MO at each corresponding time. (I,J) Expression of nr2f2 was analyzed by WISH in morphants at 24 and 36 hpf. The number at the bottom right of each panel indicates the number of embryos demonstrating the phenotype shown in the panel over the total number of embryos analyzed in a representative experiment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
apln WT control Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
WT + MO1-ptger3 standard conditions Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
bmp2b WT control Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
WT + MO1-ptger3 standard conditions Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
ccbe1 WT control Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
WT + MO1-ptger3 standard conditions Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
flt4 WT control Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
WT + MO1-ptger3 standard conditions Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
nr2f2 WT control Prim-5 posterior cardinal vein ISH
Prim-5 spinal cord ISH
Prim-5 whole organism RTPCR
Prim-25 posterior cardinal vein ISH
Prim-25 spinal cord ISH
Prim-25 whole organism RTPCR
WT + MO1-ptger3 standard conditions Prim-5 posterior cardinal vein ISH
Prim-5 spinal cord ISH
Prim-5 whole organism RTPCR
Prim-25 posterior cardinal vein ISH
Prim-25 spinal cord ISH
Prim-25 whole organism RTPCR
sox18 WT control Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
WT + MO1-ptger3 standard conditions Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
vegfc WT control Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
WT + MO1-ptger3 standard conditions Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
wnt5b WT control Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
WT + MO1-ptger3 standard conditions Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ptger3 standard conditions Prim-5 whole organism sox18 expression decreased amount, abnormal
Prim-25 posterior cardinal vein nr2f2 expression decreased amount, abnormal
Prim-25 whole organism nr2f2 expression decreased amount, abnormal
Prim-25 whole organism sox18 expression decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.