ZFIN ID: ZDB-FIG-190723-256
Iwasaki et al., 2019 - Essential role of prostaglandin E2 and the EP3 receptor in lymphatic vessel development during zebrafish embryogenesis. Scientific Reports   9:7650 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-5

Figure 5

COX1-derived PGE2 is involved in the lymphatic specification. (A) Expression of COX1, COX2a, and COX2b was analyzed by WISH at 24 hpf. (B) Zebrafish embryos were treated with Veh or SC-560 (25 μM) in the absence or presence of Sulp (10 μM) from 0 to 24 hpf, and the expression of lyve1b was analyzed by WISH at 24 hpf. The number at the bottom right of each panel indicates the number of embryos demonstrating the phenotype shown in the panel over the total number of embryos analyzed in a representative experiment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lyve1b WT standard conditions Prim-5 lymph vessel ISH
WT chemical treatment: SC560 Prim-5 lymph vessel ISH
WT chemical treatment: sulprostone, chemical treatment: SC560 Prim-5 lymph vessel ISH
ptgs1 WT standard conditions Prim-5 mesoderm ISH
ptgs2a WT standard conditions Prim-5 Not Detected trunk ISH
ptgs2b WT standard conditions Prim-5 Not Detected trunk ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: SC560 Prim-5 lymph vessel lyve1b expression decreased distribution, abnormal
Acknowledgments:
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