ZFIN Figure: Iwasaki et al., 2019, Figure 5

ZFIN ID: ZDB-FIG-190723-256

ZFIN ID: ZDB-FIG-190723-256

Iwasaki et al., 2019 - Essential role of prostaglandin E₂ and the EP3 receptor in lymphatic vessel development during zebrafish embryogenesis. Scientific Reports 9:7650 Full text @ Sci. Rep.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	lyve1b ptgs1 ptgs2a ptgs2b
Fish:	WT
Conditions:	chemical treatment: SC560 chemical treatment: sulprostone
Anatomical Terms:	lymph vessel mesoderm
Stage:	Prim-5

PHENOTYPE:

Fish:	WT
Condition:	chemical treatment: SC560
Observed In:	lymph vessel
Stage:	Prim-5

Figure 5

COX1-derived PGE₂ is involved in the lymphatic specification. (A) Expression of COX1, COX2a, and COX2b was analyzed by WISH at 24 hpf. (B) Zebrafish embryos were treated with Veh or SC-560 (25 μM) in the absence or presence of Sulp (10 μM) from 0 to 24 hpf, and the expression of lyve1b was analyzed by WISH at 24 hpf. The number at the bottom right of each panel indicates the number of embryos demonstrating the phenotype shown in the panel over the total number of embryos analyzed in a representative experiment.

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
lyve1b	WT	standard conditions	Prim-5		lymph vessel	ISH
	WT	chemical treatment: SC560	Prim-5		lymph vessel	ISH
	WT	chemical treatment: sulprostone, chemical treatment: SC560	Prim-5		lymph vessel	ISH
ptgs1	WT	standard conditions	Prim-5		mesoderm	ISH
ptgs2a	WT	standard conditions	Prim-5	Not Detected	trunk	ISH
ptgs2b	WT	standard conditions	Prim-5	Not Detected	trunk	ISH

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT	chemical treatment: SC560	Prim-5	lymph vessel lyve1b expression decreased distribution, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.